Artículo
Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
Stringa, Pablo Luis
; Romanin, David Emmanuel
; Lausada, Natalia Raquel; Papa Gobbi, Rodrigo
; Zanuzzi, Carolina Natalia
; Martín, Pedro
; Abate, Juan Cruz; Cabanne, Ana; Arnal, Nathalie
; Vecchio Dezillio, Leandro Emmanuel; Milesi, Verónica
; Portiansky, Enrique Leo
; Gondolesi, Gabriel Eduardo
; Rumbo, Martín
Fecha de publicación:
11/2017
Editorial:
Lippincott Williams & Wilkins
Revista:
Transplantation Direct
ISSN:
2373-8731
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
BACKGROUND: intestinal transplantation (ITx) faces many challenges due to the complexity of surgery and to the multiple immunological reactions that leads to the necessity of rigorous follow-up for early detection of acute cellular rejection (ACR). Our aim was to determine the kinetics of ACR using an experimental ITx model, with emphasis in the characterization of the process using different approaches, including the use of functional assays of absorptive and barrier function. METHODS: ITx in rats conducting serial sampling was performed. Clinical monitoring, graft histology, proinflammatory gene expression and nitrosative stress determination were performed. Also, glucose absorption, barrier function using ovalbumin translocation and contractile function were analyzed. RESULTS: the model used reproduced the different stages of ACR. Allogeneic ITx recipients showed signs of rejection from post-operative day (POD) 5, with increasing severity until 12 POD. Histological evaluation showed mild rejection in early sampling and severe rejection at late stages, with alterations in all graft layers. IL-6, CXCL 10, IFNg and nitrite plasmas levelsshowed behaviour coincident with histopathology. Remarkably, allogeneic grafts showed a marked alteration of glucose absorptive capacity from POD 5 that was sustained until endpoint. Coincidently, barrier function alteration was evidenced by luminal OVA translocation to serum. Contractile function was progressively impaired along ACR. CONCLUSIONS: glucose absorption and barrier function are altered at early stages of ACR, when histological alterations or gene expression changes were much subtle. This observation may provide simple evaluation tools that could be eventually translated to the clinics to contribute to early ACR diagnosis.
Palabras clave:
Xenotransplant
,
Apoptosis
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IIFP)
Articulos de INST. DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Articulos de INST. DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Articulos(INIBIOLP)
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Citación
Stringa, Pablo Luis; Romanin, David Emmanuel; Lausada, Natalia Raquel; Papa Gobbi, Rodrigo; Zanuzzi, Carolina Natalia; et al.; Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model; Lippincott Williams & Wilkins; Transplantation Direct; 3; 11; 11-2017; 1-9
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