Artículo
In Silico Analysis of Fatty Acid Desaturase Genes and Proteins in Grasses
Fecha de publicación:
28/07/2017
Editorial:
Humana Press
Revista:
Applied Biochemistry And Biotechnology
ISSN:
0273-2289
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fatty acid desaturases (FADs) catalyze the introduction of a double bond into acyl chains. Two FAD groups have been identified in plants: acyl-acyl carrier proteins (ACPs) and acyl-lipid or membrane-bound FAD. The former catalyze the conversion of 18:0 to 18:1 and to date have only been identified in plants. The latter are found in eukaryotes and bacteria and are responsible for multiple desaturations. In this study, we identified 82 desaturase gene and protein sequences from 10 grass species deposited in GenBank that were analyzed using bioinformatic approaches. Subcellular localization predictions of desaturase family revealed their localization in plasma membranes, chloroplasts, endoplasmic reticula, and mitochondria. The in silico mapping showed multiple chromosomal locations in most species. Furthermore, the presence of the characteristic histidine domains, the predicted motifs, and the finding of transmembrane regions strongly support the protein functionality. The identification of putative regulatory sites in the promotor and the expression profiles revealed the wide range of pathways in which fatty acid desaturases are involved. This study is an updated survey on desaturases of grasses that provides a comprehensive insight into diversity and evolution. This characterization is a necessary first step before considering these genes as candidates for new biotechnological approaches.
Palabras clave:
Domain Prediction
,
Grasses
,
In Silico Expression Analysis
,
Plant Desaturases
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CERZOS)
Articulos de CENTRO REC.NAT.RENOVABLES DE ZONA SEMIARIDA(I)
Articulos de CENTRO REC.NAT.RENOVABLES DE ZONA SEMIARIDA(I)
Citación
Díaz, Marina Lucía; Cuppari, Selva Yanet; Soresi, Daniela Soledad; Carrera, Alicia; In Silico Analysis of Fatty Acid Desaturase Genes and Proteins in Grasses; Humana Press; Applied Biochemistry And Biotechnology; 184; 2; 28-7-2017; 1-16
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