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Artículo

Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants

Hartman, Matias DanielIcon ; Figueroa, Carlos MariaIcon ; Arias, Diego GustavoIcon ; Iglesias, Alberto AlvaroIcon
Fecha de publicación: 01/2017
Editorial: Oxford University Press
Revista: Plant And Cell Physiology
ISSN: 0032-0781
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Glucitol, also known as sorbitol, is a major photosynthetic product in plants from the Rosaceae family. This sugar alcohol is synthesized from glucose-6-phosphate by the combined activities of aldose-6-phosphate reductase (Ald6PRase) and glucitol-6-phosphatase. In this work we show the purification and characterization of recombinant Ald6PRase from peach leaves. The recombinant enzyme was inhibited by glucose-1-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate and orthophosphate. Oxidizing agents irreversibly inhibited the enzyme and produced protein precipitation. Enzyme thiolation with oxidized glutathione protected the enzyme from insolubilization caused by diamide, while incubation with NADP+ (one of the substrates) completely prevented enzyme precipitation. Our results suggest that Ald6PRase is finely regulated to control carbon partitioning in peach leaves.
Palabras clave: Carbon Partitioning , Glucitol , Peach , Prunus Persica , Redox Regulation , Sorbitol
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/66481
DOI: https://dx.doi.org/10.1093/pcp/pcw180
URL: https://academic.oup.com/pcp/article/58/1/145/2736391
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Hartman, Matias Daniel; Figueroa, Carlos Maria; Arias, Diego Gustavo; Iglesias, Alberto Alvaro; Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants; Oxford University Press; Plant And Cell Physiology; 58; 1; 1-2017; 145-155
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