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dc.contributor.author
Pérez-Pérez, Antonio
dc.contributor.author
Gambino, Yésica Paola
dc.contributor.author
Maymo, Julieta Lorena
dc.contributor.author
Goberna, Raimundo
dc.contributor.author
Fabiani, Fernando
dc.contributor.author
Varone, Cecilia Laura
dc.contributor.author
Sánchez Margalet, Víctor
dc.date.available
2018-12-12T20:22:26Z
dc.date.issued
2010-06
dc.identifier.citation
Pérez-Pérez, Antonio; Gambino, Yésica Paola; Maymo, Julieta Lorena; Goberna, Raimundo; Fabiani, Fernando; et al.; MAPK and PI3K activities are required for leptin stimulation of protein synthesis in human trophoblastic cells; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 396; 4; 6-2010; 956-960
dc.identifier.issn
0006-291X
dc.identifier.uri
http://hdl.handle.net/11336/66369
dc.description.abstract
Leptin, the LEP gene product, is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy. Thus, we have recently described the antiapoptotic and trophic effect of leptin on choriocarcinoma cell line JEG-3, stimulating DNA and protein synthesis. We have also demonstrated the presence of leptin receptor and leptin signaling in normal human trophoblastic cells, activating JAK-STAT, PI3K and MAPK pathways. In the present work we have employed dominant negative forms of MAPK and PKB constructs to find out the signaling pathways that specifically mediates the effect of leptin on protein synthesis. As previously shown, leptin stimulates protein synthesis as assessed by 3H-leucine incorporation. However, both dominant negative forms of MAPK and PKB inhibited protein synthesis in JEG-3 choriocarcinoma cells. The inhibition of PKB and MAPK activity by transfection with the dominant negative kinases prevented the leptin stimulation of p70 S6K, which is known to be an important kinase in the regulation of protein synthesis. Moreover, leptin stimulation of phosphorylation of EIF4EBP1 and EIF4E, which allows the initiation of translation was also prevented by MAPK and PI3K dominant negative constructs. Therefore, these results demonstrate that both PI3K and MAPK are necessary to observe the effect of leptin signaling that mediates protein synthesis in choriocarcinoma cells JEG-3. © 2010 Elsevier Inc. All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Leptin
dc.subject
Placenta
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Protein Synthesis
dc.subject
Signal Transduction
dc.subject
Translation
dc.subject.classification
Salud Ocupacional
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
MAPK and PI3K activities are required for leptin stimulation of protein synthesis in human trophoblastic cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-11-22T15:28:50Z
dc.journal.volume
396
dc.journal.number
4
dc.journal.pagination
956-960
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Pérez-Pérez, Antonio. Universidad de Sevilla; España
dc.description.fil
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Goberna, Raimundo. Universidad de Sevilla; España
dc.description.fil
Fil: Fabiani, Fernando. Universidad de Sevilla; España
dc.description.fil
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España
dc.journal.title
Biochemical and Biophysical Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbrc.2010.05.031
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X10009228


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  • Articulos(IQUIBICEN) [509]
    Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES

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