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dc.contributor.author
Pavan, María Elisa
dc.contributor.author
Cairo, Fabian Martin
dc.contributor.author
Pettinari, María Julia
dc.contributor.author
Samartino, Luis Ernesto
dc.contributor.author
Brihuega, Bibiana
dc.date.available
2018-12-12T20:22:19Z
dc.date.issued
2011-03
dc.identifier.citation
Pavan, María Elisa; Cairo, Fabian Martin; Pettinari, María Julia; Samartino, Luis Ernesto; Brihuega, Bibiana; Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA); Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 34; 2; 3-2011; 135-141
dc.identifier.issn
0147-9571
dc.identifier.uri
http://hdl.handle.net/11336/66368
dc.description.abstract
Leptospirosis outbreaks occur regularly in Argentina and other South American countries, but little is known about their epidemiological relationships. Application of new molecular tools, such as the Multiple-Locus Variable-number tandem repeat Analysis (MLVA) is limited by scant available data on regional strains. We have analyzed the genetic diversity of a collection of 31 strains of Leptospira interrogans isolated in Argentina during the past five decades from humans and animals, including a strain from an environmental source and another isolated from an opossum. Genotyping was performed by MLVA using the loci VNTR4, VNTR7, VNTR9, VNTR10, VNTR19, VNTR23 and VNTR31, as described by Majed et al. [Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto. J Clin Microbiol 2005;43:539-45 [1]]. Clustering analysis revealed eight distinct MLVA genotypes, with a dominant one, genotype A. Strains with this genotype were consistently isolated since 1960, representing 55% of the total strains and spanning an extensive geographical distribution. Other seven genotypes were less frequent, and only genotypes A and Hond Utrecht IV were isolated during the last decade. Different kinds of repeat blocks for each VNTR locus were identified by sequence analysis. VNTR copy number differences among genotypes always involved only one of these blocks. MLVA patterns obtained reveal the genetic diversity and relationships between strains, and constitute the framework for the genotyping of leptospires in the region. © 2010 Elsevier Ltd.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Genotyping
dc.subject
Leptospira Interrogans
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Molecular Diversity
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Multiple-Locus Variable-Number Tandem Repeat Analysis (Mlva)
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Genotyping of Leptospira interrogans strains from Argentina by Multiple-Locus Variable-number tandem repeat Analysis (MLVA)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-11-22T15:28:15Z
dc.journal.volume
34
dc.journal.number
2
dc.journal.pagination
135-141
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Pavan, María Elisa. Biochemiq S.A.; Argentina
dc.description.fil
Fil: Cairo, Fabian Martin. Biochemiq S.A.; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Pettinari, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Universidad del Salvador; Argentina
dc.description.fil
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Universidad del Salvador; Argentina
dc.journal.title
Comparative Immunology, Microbiology and Infectious Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.cimid.2010.06.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147957110000457
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