Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility

C. guilliermondii shows intrinsic reduced echinocandin susceptibility. It harbors two polymorphisms (L633M and T634A) at the Fks1p hot-spot 1 region. Our objective was to confirm that C. guilliermondii´s reduced echinocandin susceptibility is due to those naturally occurring substitutions. We constructed a Saccharomyces cerevisiae mutant where a region of its FKS1 gene (including hot-spot 1) was replaced with that from C. guilliermondii The chimeric mutants showed 32-fold echinocandin MIC values increase confirming the hypothesis.

Palabras clave: Candida , Guilliermondii , Echinocandin , Susceptibility

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

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URI: http://hdl.handle.net/11336/66294

DOI: https://dx.doi.org/10.1128/AAC.02644-16

URL: https://aac.asm.org/content/61/5/e02644-16

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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE

Citación

Dudiuk, Catiana Beatriz; Macedo, Daiana; Leonardelli, Florencia; Theill, Laura; Cabeza, Matías Sebastián; et al.; Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 61; 5; 5-2017; 1-7

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