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dc.contributor.author
Seremeta, Katia Pamela
dc.contributor.author
Höcht, Christian
dc.contributor.author
Taira, Carlos Alberto
dc.contributor.author
Cortez Tornello, Pablo Roberto
dc.contributor.author
Abraham, Gustavo Abel
dc.contributor.author
Sosnik, Alejandro Dario
dc.date.available
2016-07-19T19:16:25Z
dc.date.issued
2015-08
dc.identifier.citation
Seremeta, Katia Pamela; Höcht, Christian; Taira, Carlos Alberto; Cortez Tornello, Pablo Roberto; Abraham, Gustavo Abel; et al.; Didanosine-loaded poly(epsilon-caprolactone) microparticles by a coaxial electrohydrodynamic atomization (CEHDA) technique; Royal Society of Chemistry; Journal Of Materials Chemistry B; 3; 1; 8-2015; 102-111
dc.identifier.issn
2050-7518
dc.identifier.uri
http://hdl.handle.net/11336/6592
dc.description.abstract
The goal of this study was to investigate the electrohydrodynamic atomization (EHDA) technology to encapsulate the water-soluble antiretroviral didanosine (ddI) within poly(epsilon-caprolactone) (PCL) particles and stabilize it in the gastric medium where it undergoes fast degradation. A preliminary study employing a one-needle setup enabled the adjustment of the critical process parameters. Then, a configuration of two concentric needles named coaxial electrohydrodynamic atomization (CEHDA) led to the formation of ddI-loaded PCL microcapsules. Scanning electron microscopy analysis showed that the microparticles were spherical and with narrow size distribution. Attenuated total reflectance/Fourier transform infrared spectroscopy confirmed that most of the drug was efficiently encapsulated within the particles, whereas differential scanning calorimetry and X-ray powder diffraction revealed that the drug was preserved mainly in crystalline form. The loading capacity was relatively high (approximately 12% w/w), and the encapsulation efficiency was approximately 100%. In vitro release assays (PBS pH ¼ 7.4) indicated that ddI was released almost completely within 2 h. Moreover, the delayed release was expected to isolate ddI from the biological fluids during the gastric transit. Finally, pharmacokinetics studies in rats showed that ddI-loaded particles lead to a statistically significant increase of the oral bioavailability of almost 4 times and a 2-fold prolongation of the half-life with respect to a ddI aqueous solution, supporting the use of CEHDA as a promising reproducible, scalable and cost-viable technology to encapsulate water-soluble drugs within polymeric particles.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hiv/Aids
dc.subject
Didanosine
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Poly(Epsilon-Caprolactone)
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Electrohydrodynamic Atomization
dc.subject.classification
Ingeniería de los Materiales
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Ingeniería de los Materiales
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Didanosine-loaded poly(epsilon-caprolactone) microparticles by a coaxial electrohydrodynamic atomization (CEHDA) technique
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-03T14:57:33Z
dc.journal.volume
3
dc.journal.number
1
dc.journal.pagination
102-111
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Seremeta, Katia Pamela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Höcht, Christian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
dc.description.fil
Fil: Taira, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Cortez Tornello, Pablo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación En Ciencia y Tecnología de Materiales (i); Argentina
dc.description.fil
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación En Ciencia y Tecnología de Materiales (i); Argentina
dc.description.fil
Fil: Sosnik, Alejandro Dario. Technion-Israel Institute of Technology. Department of Materials Science and Engineering. Group of Pharmaceutical Nanomaterials Science; Israel
dc.journal.title
Journal Of Materials Chemistry B
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/TB/c4tb00664j
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1039/C4TB00664J
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/C4TB00664J
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