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Artículo

Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation

Pomares, Maria FernandaIcon ; Salomon, Raul ArmandoIcon ; Pavlova, Olga; Severinov, Konstantin; Farias, Ricardo NorbertoIcon ; Vincent, Paula AndreaIcon
Fecha de publicación: 09/2009
Editorial: American Society for Microbiology
Revista: Applied And Environmental Microbiology
ISSN: 0099-2240
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Palabras clave: Microcin J25 , Chymotrypsin-Susceptible , Food Preservation , Potential Applicability
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/65852
URL: https://aem.asm.org/content/75/17/5734
DOI: https://dx.doi.org/10.1128/AEM.01070-09
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Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Pomares, Maria Fernanda; Salomon, Raul Armando; Pavlova, Olga; Severinov, Konstantin; Farias, Ricardo Norberto; et al.; Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation; American Society for Microbiology; Applied And Environmental Microbiology; 75; 17; 9-2009; 5734-5738
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