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dc.contributor.author
Gallo, Luciana Ines
dc.contributor.author
Dalghi, Marianela Gisela
dc.contributor.author
Clayton, Dennis R
dc.contributor.author
Ruiz, Wily G.
dc.contributor.author
Khandelwal, Puneet
dc.contributor.author
Apodaca, Gerard
dc.date.available
2018-12-04T14:36:31Z
dc.date.issued
2017-11-22
dc.identifier.citation
Gallo, Luciana Ines; Dalghi, Marianela Gisela; Clayton, Dennis R; Ruiz, Wily G.; Khandelwal, Puneet; et al.; RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells; American Physiological Society; American Journal of Physiology-cell Physiology; 314; 3; 22-11-2017; 349-365
dc.identifier.issn
0363-6143
dc.identifier.uri
http://hdl.handle.net/11336/65704
dc.description.abstract
Umbrella cells, which must maintain a tight barrier, modulate their apical surface area during bladder filling by exocytosis of an abundant, subapical pool of discoidal- and/or fusiform-shaped vesicles (DFVs). Despite the importance of this trafficking event for bladder function, the pathways the promote DFV exocytosis remain to be identified. We previously showed that DFV exocytosis depends in part on a RAB11A-RAB8A-MYO5B network, but RAB27B is also reported to be associated with DFVs, and knockout mice lacking RAB27B have fewer DFVs. However, the RAB27B requirements for DFV exocytosis, and the relationship between RAB27B and the other umbrella cell-expressed RABs remains unclear. Using a whole-bladder preparation, we observed that filling-induced exocytosis of human growth hormone-loaded DFVs was significantly inhibited when RAB27B expression was down regulated using shRNA. RAB27A was also expressed in rat urothelium; however, RAB27A-specific shRNAs did not inhibit exocytosis, and the combination of RAB27A and RAB27B shRNAs did not significantly affect DFV exocytosis more than treatment with RAB27B shRNA alone. RAB27B and RAB11A showed a small degree of overlap when quantified using SQUASSH segmentation software, and expression of dominant-active or dominant-negative mutants of RAB11A or RAB8A, or expression of a RAB11A-specific shRNA, had no significant effect on the size, number, or intensity of RAB27B-positive DFVs. Likewise, treatment with RAB27B-specific shRNA had no effect on RAB11A-positive DFV parameters. We conclude that RAB27B, but not RAB27A, regulates DFV exocytosis in bladder umbrella cells in a manner that may be parallel to the previously described RAB11A-RAB8A-MYO5B pathway.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Physiological Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Rab27b
dc.subject
Bladder
dc.subject
Exocytosis
dc.subject
Urothelium
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-23T18:10:24Z
dc.journal.volume
314
dc.journal.number
3
dc.journal.pagination
349-365
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Gallo, Luciana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.description.fil
Fil: Dalghi, Marianela Gisela. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Clayton, Dennis R. University of Pittsburgh; Estados Unidos
dc.description.fil
Fil: Ruiz, Wily G.. University of Pittsburgh; Estados Unidos
dc.description.fil
Fil: Khandelwal, Puneet. University of Pittsburgh; Estados Unidos
dc.description.fil
Fil: Apodaca, Gerard. University of Pittsburgh; Estados Unidos
dc.journal.title
American Journal of Physiology-cell Physiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.physiology.org/doi/abs/10.1152/ajpcell.00218.2017
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1152/ajpcell.00218.2017
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