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Artículo

The Ile13 residue of microcin J25 is essential for recognition by the receptor FhuA, but not by the inner membrane transporter SbmA

Socias, Sergio BenjaminIcon ; Severinov, Konstantin; Salomon, Raul ArmandoIcon
Fecha de publicación: 01/2009
Editorial: Wiley Blackwell Publishing, Inc
Revista: FEMS Microbiology Letters
ISSN: 0378-1097
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Entry of the peptide antibiotic microcin J25 (MccJ25) into target cells is mediated by the outer membrane receptor FhuA and the inner membrane protein SbmA. The latter also transports MccB17 into the cell cytoplasm. Comparison of MccJ25 and MccB17 revealed a tetrapeptide sequence (VGIG) common to both antibiotics. We speculated that this structural feature in MccJ25 could be a motif recognized by SbmA. To test this hypothesis, we used a MccJ25 variant in which the isoleucine in VGIG (position 13 in the MccJ25 sequence) was replaced by lysine (I13K). In experiments in which the FhuA receptor was bypassed, the substituted microcin showed an inhibitory activity similar to that of the wild-type peptide. Moreover, MccJ25 interfered with colicin M uptake by FhuA in a competition assay, while the I13K mutant did not. From these results, we propose that the Ile13 residue is only required for interaction with FhuA, and that VGIG is not a major recognition element by SbmA. © 2009 Federation of European Microbiological Societies.
Palabras clave: Fhua , I13k Variant , Microcin J25 , Sbma , Uptake
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/65689
DOI: https://dx.doi.org/10.1111/j.1574-6968.2009.01805.x
URL: https://academic.oup.com/femsle/article/301/1/124/615228
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Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Socias, Sergio Benjamin; Severinov, Konstantin; Salomon, Raul Armando; The Ile13 residue of microcin J25 is essential for recognition by the receptor FhuA, but not by the inner membrane transporter SbmA; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 301; 1; 1-2009; 124-129
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