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dc.contributor.author
Garcia, Daniel Asmed
dc.contributor.author
Marin, Raul Hector
dc.contributor.author
Perillo, Maria Angelica
dc.date.available
2018-12-04T13:08:06Z
dc.date.issued
2002-07
dc.identifier.citation
Garcia, Daniel Asmed; Marin, Raul Hector; Perillo, Maria Angelica; Stress-induced decrement in the plasticity of the physical properties of chick brain membranes; Taylor & Francis Ltd; Molecular Membrane Biology; 19; 3; 7-2002; 221-230
dc.identifier.issn
0968-7688
dc.identifier.uri
http://hdl.handle.net/11336/65677
dc.description.abstract
The molecular basis underlying the stress-induced increment in the density of central benzodiazepine receptor from chick forebrain, observed previously at 4°C, was studied from a biophysical perspective. The thermal dependence of [3H]flunitrazepam binding to the central benzodiazepine receptor and the supramolecular organization were studied in forebrain membranes from chicks submitted to partial water immersion. The equilibrium dissociation constants increased with temperature in membrane from both control and stressed chicks. The heat capacity values in control samples (ΔCp, CON) were significantly less negative than ΔCp STR. Changes in ΔH and ΔS between 4-37°C were greater in stressed chicks compared to control; however, the binding was exothermic and driven by enthalpy in both conditions. At 4°C, the receptor density (Bmax) was higher in stressed chicks compared to control. Such a difference was lost irreversibly upon temperature elevation, possibly due to the hysteresis between the heating and cooling behaviour of Bmax, CON and the constancy in Bmax, STR. The fluorescence anisotropy of diphenylhexatriene was higher in control samples with respect to stressed chicks below 10°C. A temperature-induced increment in protein intrinsic-fluorescence was observed only in control, and was quenched by acrylamide more easily at 4°C than at 25°C. A higher microviscosity at 4°C in control favoured more external localizations of integral proteins; at higher temperatures, tryptophan residues moved to hydrophobic membrane-regions. Changes in the membrane-organization towards more fluid states favoured the accessibility of benzodiazepine to the central benzodiazepine receptor, expressed by the higher values of Bmax found in stressed samples at low temperatures with respect to control samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Benzodiazepine Binding
dc.subject
Dph Fluorescence
dc.subject
Dynamic Quenching
dc.subject
Gabaa Receptor
dc.subject
Membrane Fluidity
dc.subject
Stress
dc.subject
Thermodynamics
dc.subject
Tryptophan Fluorescence
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Stress-induced decrement in the plasticity of the physical properties of chick brain membranes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-11-12T13:45:57Z
dc.journal.volume
19
dc.journal.number
3
dc.journal.pagination
221-230
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.journal.title
Molecular Membrane Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1080/09687680210156786
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/09687680210156786


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