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dc.contributor.author
Devine, Catherine
dc.contributor.author
Brennan, Gerard P.
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Lanusse, Carlos Edmundo
dc.contributor.author
Alvarez, Luis Ignacio
dc.contributor.author
Trudgett, Alan G.
dc.contributor.author
Hoey, Elisabeth M.
dc.contributor.author
Fairweather, Ian
dc.date.available
2018-12-03T19:37:47Z
dc.date.issued
2012-02
dc.identifier.citation
Devine, Catherine; Brennan, Gerard P.; Lanusse, Carlos Edmundo; Alvarez, Luis Ignacio; Trudgett, Alan G.; et al.; Potentiation of triclabendazole action in vivo against a triclabendazole-resistant isolate of Fasciola hepatica following its co-administration with the metabolic inhibitor, ketoconazole; Elsevier Science; Veterinary Parasitology; 184; 1; 2-2012; 37-47
dc.identifier.issn
0304-4017
dc.identifier.uri
http://hdl.handle.net/11336/65649
dc.description.abstract
An in vivo study in the laboratory rat model has been carried out to monitor morphological changes in adult Fasciola hepatica over a 4-day period resulting from co-treatment with triclabendazole (TCBZ) and ketoconazole (KTZ), a cytochrome P450 inhibitor. Rats were infected with the triclabendazole-resistant Oberon isolate of F. hepatica, dosed orally with triclabendazole at a dosage of 10. mg/kg live weight and ketoconazole at a dosage of 10. mg/kg live weight. Flukes were recovered at 24, 48, 72 and 96. h post-treatment (p.t.) and changes to fluke ultrastructure were assessed using transmission electron microscopy (TEM). Results showed an increase in the severity of changes to the fluke ultrastructure with time p.t. Swelling of the basal infolds and the associated mucopolysaccharide masses became more severe with time. Golgi complexes, if present, were greatly reduced in size and number by 96. h p.t., and sub-tegumental flooding was seen from the 72. h time-period onwards. Some sloughing of the tegumental covering over the spines was observed at 96. h p.t. The results demonstrated that the Oberon isolate is more sensitive to TCBZ action in the presence of KTZ than to TCBZ alone, reinforcing the idea that altered drug metabolism is involved in the resistance mechanism. Moreover, they support the concept that TCBZ. +. inhibitor combinations (aimed at altering drug pharmacokinetics and potentiating the action of TCBZ) could be used in the treatment of TCBZ-R populations of F. hepatica.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fasciola Hepatica
dc.subject
Ketoconazole
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Liver Fluke
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Transmission Electron Microscopy
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Triclabendazole Resistance
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Potentiation of triclabendazole action in vivo against a triclabendazole-resistant isolate of Fasciola hepatica following its co-administration with the metabolic inhibitor, ketoconazole
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-18T16:15:33Z
dc.journal.volume
184
dc.journal.number
1
dc.journal.pagination
37-47
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Devine, Catherine. Queens University; Canadá
dc.description.fil
Fil: Brennan, Gerard P.. Queens University; Canadá
dc.description.fil
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
dc.description.fil
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
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Fil: Trudgett, Alan G.. Queens University; Canadá
dc.description.fil
Fil: Hoey, Elisabeth M.. Queens University; Canadá
dc.description.fil
Fil: Fairweather, Ian. Queens University; Canadá
dc.journal.title
Veterinary Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2011.08.006
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401711005395
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