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dc.contributor.author Menendez-Bravo, Simón Matías
dc.contributor.author Paganini, Julián Andrés
dc.contributor.author Avignone Rossa, Claudio
dc.contributor.author Gramajo, Hugo Cesar
dc.contributor.author Arabolaza, Ana Lorena
dc.date.available 2018-11-28T20:10:16Z
dc.date.issued 2017-08-02
dc.identifier.citation Menendez-Bravo, Simón Matías; Paganini, Julián Andrés; Avignone Rossa, Claudio; Gramajo, Hugo Cesar; Arabolaza, Ana Lorena; Identification of FadAB complexes involved in fatty acid β-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing strain; Frontiers Research Foundation; Frontiers in Microbiology; 8; AUG; 2-8-2017; 1-17; 1428
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/11336/65528
dc.description.abstract Oleaginous microorganisms represent possible platforms for the sustainable production of oleochemicals and biofuels due to their metabolic robustness and the possibility to be engineered. Streptomyces coelicolor is among the narrow group of prokaryotes capable of accumulating triacylglycerol (TAG) as carbon and energy reserve. Although the pathways for TAG biosynthesis in this organism have been widely addressed, the set of genes required for their breakdown have remained elusive so far. Here, we identified and characterized three gene clusters involved in the β-oxidation of fatty acids (FA). The role of each of the three different S. coelicolor FadAB proteins in FA catabolism was confirmed by complementation of an Escherichia coliΔfadBA mutant strain deficient in β-oxidation. In S. coelicolor, the expression profile of the three gene clusters showed variation related with the stage of growth and the presence of FA in media. Flux balance analyses using a corrected version of the current S. coelicolor metabolic model containing detailed TAG biosynthesis reactions suggested the relevance of the identified fadAB genes in the accumulation of TAG. Thus, through the construction and analysis of fadAB knockout mutant strains, we obtained an S. coelicolor mutant that showed a 4.3-fold increase in the TAG content compared to the wild type strain grown under the same culture conditions.
dc.format application/pdf
dc.language.iso eng
dc.publisher Frontiers Research Foundation
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject BETA-OXIDATION OF FATTY ACIDS
dc.subject CHEMOSTAT SYSTEM
dc.subject NEUTRAL LIPID
dc.subject STREPTOMYCES COELICOLOR
dc.subject TRIACYLGLYCEROL ACCUMULATION
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Identification of FadAB complexes involved in fatty acid β-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing strain
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-10-16T19:04:41Z
dc.journal.volume 8
dc.journal.number AUG
dc.journal.pagination 1-17; 1428
dc.journal.pais Suiza
dc.description.fil Fil: Menendez-Bravo, Simón Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil Fil: Paganini, Julián Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil Fil: Avignone Rossa, Claudio. University of Surrey; Reino Unido
dc.description.fil Fil: Gramajo, Hugo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil Fil: Arabolaza, Ana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.journal.title Frontiers in Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3389/fmicb.2017.01428
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2017.01428/full
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)