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Artículo

Bordetella parapertussis circumvents neutrophil extracellular bactericidal mechanisms

Gorgojo, Juan PabloIcon ; Scharrig Fernandez, Maria EmiliaIcon ; Gomez, Ricardo MartinIcon ; Harvill, Eric; Rodriguez, Maria EugeniaIcon
Fecha de publicación: 01/2017
Editorial: Public Library of Science
Revista: Plos One
ISSN: 1932-6203
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnologías de la Salud

Resumen

B. parapertussis is a whooping cough etiological agent with the ability to evade the immune response induced by pertussis vaccines. We previously demonstrated that in the absence of opsonic antibodies B. parapertussis hampers phagocytosis by neutrophils and macrophages and, when phagocytosed, blocks intracellular killing by interfering with phagolysosomal fusion. But neutrophils can kill and/or immobilize extracellular bacteria through nonphagocytic mechanisms such as degranulation and neutrophil extracellular traps (NETs). In this study we demonstrated that B. parapertussis also has the ability to circumvent these two neutrophil extracellular bactericidal activities. The lack of neutrophil degranulation was found dependent on the O antigen that targets the bacteria to cell lipid rafts, eventually avoiding the fusion of nascent phagosomes with specific and azurophilic granules. IgG opsonization overcame this inhibition of neutrophil degranulation. We further observed that B. parapertussis did not induce NETs release in resting neutrophils and inhibited NETs formation in response to phorbol myristate acetate (PMA) stimulation by a mechanism dependent on adenylate cyclase toxin (CyaA)-mediated inhibition of reactive oxygen species (ROS) generation. Thus, B. parapertussis modulates neutrophil bactericidal activity through two different mechanisms, one related to the lack of proper NETs-inducer stimuli and the other one related to an active inhibitory mechanism. Together with previous results these data suggest that B. parapertussis has the ability to subvert the main neutrophil bactericidal functions, inhibiting efficient clearance in non-immune hosts.
Palabras clave: Degranulation , Neutrphil Extracellular Traps , Immune Evasion , Bordetella , Host Pathogen Interaction
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/65212
DOI: http://dx.doi.org/10.1371/journal.pone.0169936
URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169936
Colecciones
Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Gorgojo, Juan Pablo; Scharrig Fernandez, Maria Emilia; Gomez, Ricardo Martin; Harvill, Eric; Rodriguez, Maria Eugenia; Bordetella parapertussis circumvents neutrophil extracellular bactericidal mechanisms; Public Library of Science; Plos One; 12; 1; 1-2017; 1-25; e0169936
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