Artículo
Molecular tools for cryptic Candida species identification with applications in a clinical laboratory
Gamarra, Soledad; Dudiuk, Catiana Beatriz
; Mancilla, Estefanía; Vera Garate, María Verónica; Guerrero, Sergio Adrian
; Garcia, Guillermo Manuel
Fecha de publicación:
03/2013
Editorial:
Wiley
Revista:
Biochemistry and Molecular Biology Education
ISSN:
1470-8175
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Candida spp. includes more than 160 species but only 20 species pose clinical problems. C. albicans and C. parapsilosis account for more than 75% of all the fungemias worldwide. In 1995 and 2005, one C. albicans and two C. parapsilosis-related species were described, respectively. Using phenotypic traits, the identification of these newly described species is inconclusive or impossible. Thus, molecular-based procedures are mandatory. In the proposed educational experiment we have adapted different basic molecular biology techniques designed to identify these species including PCR, multiplex PCR, PCR-based restriction endonuclease analysis and nuclear ribosomal RNA amplification. During the classes, students acquired the ability to search and align gene sequences, design primers, and use bioinformatics software. Also, in the performed experiments, fungal molecular taxonomy concepts were introduced and the obtained results demonstrated that classic identification (phenotypic) in some cases needs to be complemented with molecular-based techniques. As a conclusion we can state that we present an inexpensive and well accepted group of classes involving important concepts that can be recreated in any laboratory.
Palabras clave:
Candida
,
Taxonomia
,
Molecular
,
Metodos
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Gamarra, Soledad; Dudiuk, Catiana Beatriz; Mancilla, Estefanía; Vera Garate, María Verónica; Guerrero, Sergio Adrian; et al.; Molecular tools for cryptic Candida species identification with applications in a clinical laboratory; Wiley; Biochemistry and Molecular Biology Education; 41; 3; 3-2013; 180-186
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