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dc.contributor.author
Santarossa, Debora Gimena
dc.contributor.author
Fernandez, Liliana Patricia
dc.date.available
2018-11-20T17:41:16Z
dc.date.issued
2017-09-09
dc.identifier.citation
Santarossa, Debora Gimena; Fernandez, Liliana Patricia; Development of on-line spectrofluorimetric methodology for selenium monitoring in foods and biological fluids using Chrome azurol S quenching; Elsevier Science; Talanta; 172; 9-9-2017; 31-36
dc.identifier.issn
0039-9140
dc.identifier.uri
http://hdl.handle.net/11336/64761
dc.description.abstract
A novel, simple and accurate on-line green methodology for Se(IV) monitoring by molecular fluorescence has been developed. Because the analyte does not exhibit fluorescence, the organic dye Chrome azurol S (CAS) has been chosen to allow detection. The effect of metal-quenching on CAS excitation and emission conditions (λex=300 nm; λem=407 nm) was used as criterion for analyte quantification in presence of sodium cholate bile salt (NaC). The quenching mechanism was explored, and it can be classified as a collisional type with a Stern-Volmer constant value of 3.0×107 mol L−1. To improve the sampling rate, minimize the reagent consumption and generated wastes, an on-line configuration was designed. Experimental variables that affect the fluorimetric sensitivity were optimized using uni-variation assays. Under optimal experimental conditions, the limit of detection was 0.27 µg L−1 with a lineal range for Se(IV) concentration from 0.84 to 6.00 µg L−1. The developed methodology is low cost and fast sampling, allowing Se(IV) quantification in the presence of other common ions. Bulbous vegetables and biological samples were successfully analyzed with an average recovery close to 100%.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Selenium
dc.subject
Fi-Spectrofluorometry
dc.subject
Chrome Azurol S
dc.subject
Sodium Cholate
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Development of on-line spectrofluorimetric methodology for selenium monitoring in foods and biological fluids using Chrome azurol S quenching
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-23T17:38:54Z
dc.journal.volume
172
dc.journal.pagination
31-36
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.journal.title
Talanta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0039914017305295
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.talanta.2017.05.013
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