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dc.contributor.author
Garcia, Daniel Asmed
dc.contributor.author
Perillo, Maria Angelica
dc.date.available
2018-11-15T14:53:23Z
dc.date.issued
2001-01-15
dc.identifier.citation
Garcia, Daniel Asmed; Perillo, Maria Angelica; Flunitrazepam induces geometrical changes at the lipid-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 20; 1; 15-1-2001; 63-72
dc.identifier.issn
0927-7765
dc.identifier.uri
http://hdl.handle.net/11336/64526
dc.description.abstract
Flunitrazepam (FNTZ) effects on molecular packing and surface curvature in artificial model membranes were investigated. FNTZ, from the subphase under dipalmitoylphosphatidylcholine (dpPC) monolayers at the air-water interface, expanded the surface pressure-area isotherm and induced an increment in the limiting area; in this conditions, the collapse pressure of dpPC decreased, indicating a lowering in the stability of the monolayer. Thermodynamic-geometric correlations based on molecular parameters predicted a decrement in the aggregation number and stability, and an increase in the curvature of the self-aggregated structure of dpPC in aqueous medium in the presence of FNTZ. Accordingly, negative-staining electron microscopy of dpPC aqueous dispersions showed that the mean diameter of dpPC vesicles decreased 2 and 2.87 times in the presence of 10 nM and 50 μM FNTZ, respectively, compared with control samples. The release of a soluble marker entrapped in dpPC liposomes increased slightly respect to the control in the presence of FNTZ. In dpPC-dpPE mixed liposomes 50 μM FNTZ induced a decrement in the amount of the aminophospholipid exposed to the outer monolayer. Concluding, an FNTZ-induced expansion of dpPC-water interface region affected the constraints imposed on the lipid-water system by the molecular geometry, interacting free energies and entropy that determine the shape of a multimolecular structure. In liposomes composed of a pure phospholipid, the bilayer expansion leaded, through a structure instability, to reduce the liposome size; in mixed liposomes, phospholipid molecules translocation could be observed as another compensating mechanism of the initial perturbation. These results may be relevant for understanding benzodiazepines' effects non-mediated by membrane receptors. (C) 2001 Elsevier Science B.V.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
[3h]Gaba Entrapment
dc.subject
Aminophospholipid Translocation
dc.subject
Dipalmitoylphosphatidylcholine Self-Assembly
dc.subject
Electron Microscopy
dc.subject
Flunitrazepam
dc.subject
Monomolecular Layers
dc.subject
Multilamellar Vesicles
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Flunitrazepam induces geometrical changes at the lipid-water interface
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-11-12T13:46:38Z
dc.journal.volume
20
dc.journal.number
1
dc.journal.pagination
63-72
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.journal.title
Colloids and Surfaces B: Biointerfaces
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776500001715
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/S0927-7765(00)00171-5


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