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dc.contributor.author
Marcon, Bruna H.
dc.contributor.author
Holetz, Fabíola B.
dc.contributor.author
Eastman, Guillermo
dc.contributor.author
Origa Alves, Ana Carolina
dc.contributor.author
Amorós, Mariana Andrea
dc.contributor.author
de Aguiar, Alessandra Melo
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Rebelatto, Carmen K.
dc.contributor.author
Brofman, Paulo R.S.
dc.contributor.author
Sotelo-Silveira, Jose
dc.contributor.author
Dallagiovanna, Bruno
dc.date.available
2018-11-14T19:16:25Z
dc.date.issued
2017-12
dc.identifier.citation
Marcon, Bruna H.; Holetz, Fabíola B.; Eastman, Guillermo; Origa Alves, Ana Carolina; Amorós, Mariana Andrea; et al.; Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells; Elsevier Science; Stem Cell Research; 25; 12-2017; 191-201
dc.identifier.issn
1873-5061
dc.identifier.uri
http://hdl.handle.net/11336/64505
dc.description.abstract
Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Adipogenesis
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Hascs
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Ribosome Profiling
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Translation
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Otras Ciencias Biológicas
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-11-12T13:22:42Z
dc.journal.volume
25
dc.journal.pagination
191-201
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Marcon, Bruna H.. Fundación Oswaldo Cruz; Brasil
dc.description.fil
Fil: Holetz, Fabíola B.. Fundación Oswaldo Cruz; Brasil
dc.description.fil
Fil: Eastman, Guillermo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
dc.description.fil
Fil: Origa Alves, Ana Carolina. Fundación Oswaldo Cruz; Brasil
dc.description.fil
Fil: Amorós, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: de Aguiar, Alessandra Melo. Fundación Oswaldo Cruz; Brasil
dc.description.fil
Fil: Rebelatto, Carmen K.. Pontificia Universidade Catolica Do Parana; Brasil
dc.description.fil
Fil: Brofman, Paulo R.S.. Pontificia Universidade Catolica Do Parana; Brasil
dc.description.fil
Fil: Sotelo-Silveira, Jose. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay. Universidad de la República; Uruguay
dc.description.fil
Fil: Dallagiovanna, Bruno. Fundación Oswaldo Cruz; Brasil
dc.journal.title
Stem Cell Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.scr.2017.10.027
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1873506117302301
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