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Artículo

Antifungal photosensitive activity of Porophyllum obscurum (Spreng.) DC.: Correlation of the chemical composition of the hexane extract with the bioactivity

Postigo, AgustinaIcon ; Funes, Matías DanielIcon ; Petenatti, Elisa Margarita; Bottai, Hebe; Pacciaroni, Adriana del Valle; Sortino, Maximiliano AndrésIcon
Fecha de publicación: 12/2017
Editorial: Elsevier Science
Revista: Photodiagnosis Photodynamic Therapy
ISSN: 1572-1000
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

We report Porophyllum obscurum as a source of new photosensitizers with potential use in Photodynamic Therapy as an alternative for oropharyngeal candidiasis treatment. The antifungal photosensitive activity of different extracts from P. obscurum was evaluated by using microdilution and bioautographic assays. The Minimum Fungicidal Concentration for hexanic extract under UV-A irradiation was 0.98 μg/mL, but it was inactive in experiments without irradiation. The bioassay-guided fractionation of this extract led to the isolation of four thiophenes responsible for the photosensitive activity: 2,2′:5′2″terthiophene, 5-(3-buten-1-ynyl)-2,2′-bithiophene, 5-(4-acetoxy-1-butenyl)-2,2′- bithiophene and 5-(4-hydroxy-1-butenyl)-2,2′- bithiophene, with Minimum Fungicidal Concentrations ranging 0.24-7.81 μg/mL under UV-A irradiation. The activity of the hexanic extract was evaluated against 25 clinical strains of Candida spp. isolates as etiological agents of oropharyngeal candidiasis. No differences in susceptibility were observed in strains resistant and susceptible to conventional antifungal drugs. Qualitative and quantitative chemical analyses of seven samples of P. obscurum collected in four different phenological stages were carried out showing that full flowering stage possesses the highest thiophenes content. These data also allowed us to establish a correlation between the thiophene composition of the different extracts and their antifungal photosensitive activity, according to a second order polynomial model with the equation: y = 11.2603-0.6831*x + 0.0108*x2. The thiophenes isolated were the responsible of antifungal photosensitive activity and can be used for the future standardization of the extract. Results showed that P. obscurum hexanic extract could be potentially developed as an Herbal Medicinal Product to be applied as a photosensitizer in Photodynamic Therapy.
Palabras clave: Candida , Composition Activity Correlation , Oropharyngeal Candidiasis , Photodynamic Therapy , Porophyllum Obscurum , Tiophenes
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/64454
DOI: https://doi.org/10.1016/j.pdpdt.2017.10.023
URL: https://www.sciencedirect.com/science/article/pii/S157210001730368X
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Articulos(INTEQUI)
Articulos de INST. DE INVEST. EN TECNOLOGIA QUIMICA
Citación
Postigo, Agustina; Funes, Matías Daniel; Petenatti, Elisa Margarita; Bottai, Hebe; Pacciaroni, Adriana del Valle; et al.; Antifungal photosensitive activity of Porophyllum obscurum (Spreng.) DC.: Correlation of the chemical composition of the hexane extract with the bioactivity; Elsevier Science; Photodiagnosis Photodynamic Therapy; 20; 12-2017; 263-272
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