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dc.contributor.author
Michiels, Maria Soledad  
dc.contributor.author
del Valle, Juana Cristina  
dc.contributor.author
Lopez Mañanes, Alejandra Antonia  
dc.date.available
2018-11-13T21:39:36Z  
dc.date.issued
2017-02-05  
dc.identifier.citation
Michiels, Maria Soledad; del Valle, Juana Cristina; Lopez Mañanes, Alejandra Antonia; Trypsin and N-aminopeptidase (APN) activities in the hepatopancreas of an intertidal euryhaline crab: Biochemical characteristics and differential modulation by histamine and salinity; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 204; 5-2-2017; 228-235  
dc.identifier.issn
1095-6433  
dc.identifier.uri
http://hdl.handle.net/11336/64423  
dc.description.abstract
No studies are available about biochemical characteristics and modulation (i.e. by endogenous and/or environmental cues) of trypsin (a key digestive endoprotease) in hepatopancreas of intertidal euryhaline crabs neither on the possible concomitant modulation of key ectoproteases such as aminopeptidase-N (APN) involved in final steps of protein digestion. Furthermore, nothing is still known in decapods crustaceans about the role of histamine as primary chemical messenger for modulation of main components of digestive process (i.e. proteases). We determined biochemical characteristics and investigated the effect of histamine injections; of histamine in vitro and of acclimation of individuals to low and high salinity on trypsin and aminopeptidase-N (APN) activities in the hepatopancreas of the euryhaline crab Cyrtograpsus angulatus (Dana 1851). Trypsin activity was maximal at pH 7.4 and at 45 °C. APN activity increased from pH 6.6 to 7.6–9.0 and was maintained high at 37–45 °C. Both activities exhibited Michaelis-Menten kinetics (apparent Km: trypsin = 0.36 mM; APN = 0.07 mM). The injection of 10− 4 M histamine decreased trypsin activity (about 40%) in hepatopancreas while did not affect APN activity. Similarly, in vitro 10-4 M histamine decreased trypsin activity (about 52%) in hepatopancreas but not APN activity. Trypsin activity in the hepatopancreas was not affected by acclimation of crabs to low (10 psu) or high (40 psu) environmental salinity while APN activity was increased (about 200%) in 10 psu. The results show the differential modulation of trypsin and APN by distinct cues and point to histamine as modulator of intracellular trypsin by direct action on the hepatopancreas.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Ectoprotease  
dc.subject
Endoprotease  
dc.subject
Environment  
dc.subject
Hepatopancreas  
dc.subject
Histamine  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Trypsin and N-aminopeptidase (APN) activities in the hepatopancreas of an intertidal euryhaline crab: Biochemical characteristics and differential modulation by histamine and salinity  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-10-23T14:11:07Z  
dc.identifier.eissn
1531-4332  
dc.journal.volume
204  
dc.journal.pagination
228-235  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Michiels, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina  
dc.description.fil
Fil: del Valle, Juana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina  
dc.description.fil
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina  
dc.journal.title
Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1095643316302859  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.cbpa.2016.12.003