Mostrar el registro sencillo del ítem
dc.contributor.author
Rossin, Maria Alejandra
dc.contributor.author
de Francesco, Pablo Nicolás
dc.contributor.author
Timi, Juan Tomas
dc.date.available
2018-11-12T13:28:54Z
dc.date.issued
2017-08-19
dc.identifier.citation
Rossin, Maria Alejandra; de Francesco, Pablo Nicolás; Timi, Juan Tomas; Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea); Springer; Parasitology Research; 116; 10; 19-8-2017; 2813-2819
dc.identifier.issn
0932-0113
dc.identifier.uri
http://hdl.handle.net/11336/64212
dc.description.abstract
Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabitats. Indeed, diagnostic characteristics as sclerites and male copulatory complex are generally less than 100-μm long and are essential for taxonomic description and identification of species. Here, a combination of simple and routine methods for three-dimensional morphological studies on hard structures is proposed for dactylogirids: SDS treatment for clarification of specimens and enzymatic digestion with proteinase K for freeing sclerotized structures, followed by laser confocal microscopy. This method is applicable to fresh or fixed specimens and does not require staining or dehydration. Indeed, stable autofluorescence emission is detectable at 500?530 nm for bars, anchors, and male copulatory complex when excited by argon laser. Advantages of this protocol over previous methodologies for taking laser confocal images are discussed. Open access software for image processing was used for three-dimensional reconstruction of sclerotized structures generating models and full 360° rotation videos.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Monogenea
dc.subject
Sds Treatment
dc.subject
Proteinase K Treatment
dc.subject
Laser Confocal Images
dc.subject
Autofluorescence
dc.subject
Three-Dimensional Reconstruction
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-22T21:50:38Z
dc.identifier.eissn
1432-1955
dc.journal.volume
116
dc.journal.number
10
dc.journal.pagination
2813-2819
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Rossin, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
dc.description.fil
Fil: de Francesco, Pablo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
dc.description.fil
Fil: Timi, Juan Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
dc.journal.title
Parasitology Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s00436-017-5591-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00436-017-5591-y
Archivos asociados