Artículo
Histone methyltransferase 1 regulates the encystation process in the parasite Giardia lamblia
Salusso, Agostina
; Zlocowski, Natacha
; Mayol, Gonzalo Federico
; Zamponi, Nahuel
; Ropolo, Andrea Silvana
Fecha de publicación:
12/06/2017
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Febs Journal
ISSN:
1742-464X
e-ISSN:
1742-4658
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In eukaryotes, histone lysine methylation is associated with either active or repressed chromatin states, depending on the status of methylation. Even when the amino-terminus of Giardia lamblia histones diverges from other organisms, these regions contain lysine residues that are potential targets for methylation. When we examined the role of the histone methyltransferase 1 (HMT1) in the regulation of the encystation process by giardial histone methyltransferase 1 (GlHMT1) overexpression or downregulation, we observed an increase or a decrease in cyst production, respectively, compared to wild-type trophozoites. A time-lapse analysis of encystation showed that overexpression of GlHMT1 induced an earlier and faster process than in wild-type cells together with an upregulation of mRNA expression of cyst wall proteins. Subcellular localization studies indicated that GlHMT1-hemaglutinin was mainly associated with the nuclear and perinuclear region in both growing and encysting parasites, in agreement with bioinformatics analyses showing that GlHMT-1 possesses nuclear localization signals in addition to the classical SU(var)3-9, Enhancer-of-Zeste, Trithorax (SET), and post-SET domains. Altogether, these findings suggest that the function of HMT1 is critical for the success and timing of the encystation process, and reinforce the idea that epigenetic marks are critical for cyst formation in G. lamblia.
Palabras clave:
Cysts
,
Epigenetic
,
Giardia
,
Methylation
,
Parasite
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Salusso, Agostina; Zlocowski, Natacha; Mayol, Gonzalo Federico; Zamponi, Nahuel; Ropolo, Andrea Silvana; Histone methyltransferase 1 regulates the encystation process in the parasite Giardia lamblia; Wiley Blackwell Publishing, Inc; Febs Journal; 284; 15; 12-6-2017; 2396-2409
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