Artículo
Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
Rodríguez, Fernanda Mariel
; Salvetti, Natalia Raquel
; Colombero, M.; Stangaferro, M.; Barbeito, Claudio Gustavo
; Ortega, Hugo Hector
; Rey, Florencia
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Fecha de publicación:
05/2013
Editorial:
Elsevier
Revista:
Animal Reproduction Science
ISSN:
0378-4320
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P menor 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
Palabras clave:
Cattle
,
Cystic Ovarian Disease
,
Insulin-Like Growth Factor
,
Ovaries
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(ICIVET-LITORAL)
Articulos de INST. DE CIENCIAS VETERINARIAS DEL LITORAL
Articulos de INST. DE CIENCIAS VETERINARIAS DEL LITORAL
Citación
Rodríguez, Fernanda Mariel; Salvetti, Natalia Raquel; Colombero, M.; Stangaferro, M.; Barbeito, Claudio Gustavo; et al.; Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease; Elsevier; Animal Reproduction Science; 140; 1-2; 5-2013; 14-25
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