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dc.contributor.author
Fagali, Natalia Soledad  
dc.contributor.author
Grillo, Claudia Alejandra  
dc.contributor.author
Puntarulo, Susana Ángela  
dc.contributor.author
Fernandez Lorenzo, Monica Alicia  
dc.date.available
2018-11-08T15:03:17Z  
dc.date.issued
2017-12  
dc.identifier.citation
Fagali, Natalia Soledad; Grillo, Claudia Alejandra; Puntarulo, Susana Ángela; Fernandez Lorenzo, Monica Alicia; Is there any difference in the biological impact of soluble and insoluble degradation products of iron-containing biomaterials?; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 160; 12-2017; 238-246  
dc.identifier.issn
0927-7765  
dc.identifier.uri
http://hdl.handle.net/11336/63970  
dc.description.abstract
The interactions that could be built between the biomaterials and tissue- microenvironments are very complex, especially in case of degradable metals that generate a broad variety of degradation products. The interfacial problems are particularly relevant for Fe-based materials that have been proposed for the development of biodegradable implants. The cell metabolism could be affected by the accumulation of insoluble Fe-containing degradation products that has been observed in vitro and in vivo as a coarse granular brownish material around the implant. However, the relative importance of each Fe-species (soluble and insoluble) on the cellular behavior of the surrounding cells, particularly on the generation of reactive species (RS), is not completely elucidated. The aim of this study is to evaluate the processes occurring at the Fe-biomaterial/cells interfacial region, and to discriminate the effects of soluble and insoluble corrosion products released by the bulk metal (Fe- microparticles (Fe0p) or Fe0 ring) on the adjacent cells, mainly in relation to RS generation. With this purpose Fe0p and Fe0 ring were incubated with fibroblast cells (BALB/c 3T3 line) for 24 and 48 h periods. Then different techniques were used, such as the dichlorofluorescein diacetate assay (DCFH2-DA) for detection of RS, acridine orange dye for cell viability, total protein content determinations, Prussian Blue staining and TEM observations. To individualize the effects of soluble and insoluble species, independent experiments with Fe3+-salts were performed. Overall data indicate that RS generation by cells exposed to the degradation products of Fe-based biomaterials is more dependent on the presence of insoluble products than on soluble Fe species.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Biodegradable  
dc.subject
Biomaterials  
dc.subject
Interface  
dc.subject
Iron  
dc.subject
Reactive Species  
dc.subject.classification
Otras Biotecnologías de la Salud  
dc.subject.classification
Biotecnología de la Salud  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Is there any difference in the biological impact of soluble and insoluble degradation products of iron-containing biomaterials?  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-10-22T22:31:48Z  
dc.journal.volume
160  
dc.journal.pagination
238-246  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina  
dc.description.fil
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina  
dc.description.fil
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina  
dc.description.fil
Fil: Fernandez Lorenzo, Monica Alicia. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina  
dc.journal.title
Colloids and Surfaces B: Biointerfaces  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.colsurfb.2017.09.032  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776517306100