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dc.contributor.author
Thoke, Henrik S.
dc.contributor.author
Thorsteinsson, Sigmundur
dc.contributor.author
Stock, Roberto P.
dc.contributor.author
Bagatolli, Luis Alberto
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dc.contributor.author
Olsen, Lars F.
dc.date.available
2018-11-08T13:30:35Z
dc.date.issued
2017-12-24
dc.identifier.citation
Thoke, Henrik S.; Thorsteinsson, Sigmundur; Stock, Roberto P.; Bagatolli, Luis Alberto; Olsen, Lars F.; The dynamics of intracellular water constrains glycolytic oscillations in Saccharomyces cerevisiae; Nature Publishing Group; Scientific Reports; 7; 1; 24-12-2017; 16250
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/63941
dc.description.abstract
We explored the dynamic coupling of intracellular water with metabolism in yeast cells. Using the polarity-sensitive probe 6-acetyl-2-dimethylaminonaphthalene (ACDAN), we show that glycolytic oscillations in the yeast S. cerevisiae BY4743 wild-type strain are coupled to the generalized polarization (GP) function of ACDAN, which measures the physical state of intracellular water. We analysed the oscillatory dynamics in wild type and 24 mutant strains with mutations in many different enzymes and proteins. Using fluorescence spectroscopy, we measured the amplitude and frequency of the metabolic oscillations and ACDAN GP in the resting state of all 25 strains. The results showed that there is a lower and an upper threshold of ACDAN GP, beyond which oscillations do not occur. This critical GP range is also phenomenologically linked to the occurrence of oscillations when cells are grown at different temperatures. Furthermore, the link between glycolytic oscillations and the ACDAN GP value also holds when ATP synthesis or the integrity of the cell cytoskeleton is perturbed. Our results represent the first demonstration that the dynamic behaviour of a metabolic process can be regulated by a cell-wide physical property: the dynamic state of intracellular water, which represents an emergent property.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Intracelullar Water Dynamics
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Acdan
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Glycolytic Oscillations
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The dynamics of intracellular water constrains glycolytic oscillations in Saccharomyces cerevisiae
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-22T15:52:02Z
dc.journal.volume
7
dc.journal.number
1
dc.journal.pagination
16250
dc.journal.pais
Reino Unido
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dc.journal.ciudad
LOndres
dc.description.fil
Fil: Thoke, Henrik S.. Center for Biomembrane Physics; Dinamarca. University of Southern Denmark; Dinamarca
dc.description.fil
Fil: Thorsteinsson, Sigmundur. University of Southern Denmark; Dinamarca
dc.description.fil
Fil: Stock, Roberto P.. University of Southern Denmark; Dinamarca
dc.description.fil
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Center for Biomembrane Physics; Dinamarca. Yachay EP and Yachay Tech; Ecuador
dc.description.fil
Fil: Olsen, Lars F.. Center for Biomembrane Physics; Dinamarca. University of Southern Denmark; Dinamarca
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1038/s41598-017-16442-x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-16442-x
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