Artículo
Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform
Camara, Maria de Los Milagros
; Bouvier, Leon Alberto
; Canepa, Gaspar Exequiel
; Miranda, Mariana Reneé
; Pereira, Claudio Alejandro
Fecha de publicación:
07/02/2013
Editorial:
Public Library Science
Revista:
Plos Neglected Tropical Diseases
ISSN:
1935-2735
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Trypanosoma cruzi, the etiological agent of Chagas’ disease, is an early divergent eukaryote in which control of gene expression relies mainly in post-transcriptional mechanisms. Transcription levels are globally up and down regulated during the transition between proliferating and non-proliferating life-cycle stages. In this work we characterized a nuclear adenylate kinase isoform (TcADKn) that is involved in ribosome biogenesis. Nuclear adenylate kinases have been recently described in a few organisms, being all related to RNA metabolism. Depending on active transcription and translation, TcADKn localizes in the nucleolus or the cytoplasm. A non-canonical nuclear localization signal was mapped towards the Nterminal of the protein, being the phosphate-binding loop essential for its localization. In addition, TcADKn nuclear exportation depends on the nuclear exportation adapter CRM1. TcADKn nuclear shuttling is governed by nutrient availability, oxidative stress and by the equivalent in T. cruzi of the mammalian TOR (Target of Rapamycin) pathway. One of the biological functions of TcADKn is ribosomal 18S RNA processing by direct interaction with ribosomal protein TcRps14.
Finally, TcADKn expression is regulated by its 39 UTR mRNA. Depending on extracellular conditions, cells modulate protein translation rates regulating ribosome biogenesis and nuclear adenylate kinases are probably key components in these processes.
Palabras clave:
Trypanosoma Cruzi
,
Chagas Disease
,
Adenylate Kinase
,
Ribosome Processing
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IDIM)
Articulos de INST.DE INVEST.MEDICAS
Articulos de INST.DE INVEST.MEDICAS
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Canepa, Gaspar Exequiel; Miranda, Mariana Reneé; Pereira, Claudio Alejandro; Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform; Public Library Science; Plos Neglected Tropical Diseases; 7; 7-2-2013; 2044-2047;
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