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Artículo

Magnetic nanoparticles for drug targeting: From design to insights into systemic toxicity. Preclinical evaluation of hematological, vascular and neurobehavioral toxicology

Agotegaray, Mariela AlejandraIcon ; Campelo, Adrián EstebanIcon ; Zysler, Roberto DanielIcon ; Gumilar, Fernanda AndreaIcon ; Bras, Cristina LilianaIcon ; Gandini, Norberto ArielIcon ; Minetti, A.; Massheimer, Virginia LauraIcon ; Lassalle, Verónica LeticiaIcon
Fecha de publicación: 04/2017
Editorial: Royal Society of Chemistry
Revista: Biomaterials Science
ISSN: 2047-4849
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnologías de la Salud

Resumen

A simple two-step drug encapsulation method was developed to obtain biocompatible magnetic nanocarriers for the potential targeted treatment of diverse diseases. The nanodevice consists of a magnetite core coated with chitosan (Chit@MNPs) as a platform for diclofenac (Dic) loading as a model drug (Dic-Chit@MNPs). Mechanistic and experimental conditions related to drug incorporation and quantification are further addressed. This multi-disciplinary study aims to elucidate the toxicological impact of the MNPs at hematological, vascular, neurological and behavioral levels. Blood compatibility assays revealed that MNPs did not affect either erythrosedimentation rates or erythrocyte integrity at the evaluated doses (1, 10 and 100 μg mL-1). A microscopic evaluation of blood smears indicated that MNPs did not induce morphological changes in blood cells. Platelet aggregation was not affected by MNPs either and just a slight diminution was observed with Dic-Chit@MNPs, an effect possibly due to diclofenac. The examined formulations did not exert cytotoxicity on rat aortic endothelial cells and no changes in cell viability or their capacity to synthesize NO were observed. Behavioral and functional nervous system parameters in a functional observational battery were assessed after a subacute treatment of mice with Chit@MNPs. The urine pools of the exposed group were decreased. Nephritis and an increased number of megakaryocytes in the spleen were observed in the histopathological studies. Sub-acute exposure to Chit@MNPs did not produce significant changes in the parameters used to evaluate neurobehavioral toxicity. The aspects focused on within this manuscript are relevant at the pre-clinical level providing new and novel knowledge concerning the biocompatibility of magnetic nanodevices for biomedical applications.
Palabras clave: Magnetic Nanoparticles , Drug Targeting , Chitosan , Vascular
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/63364
DOI: http://dx.doi.org/10.1039/c6bm00954a
URL: https://pubs.rsc.org/en/Content/ArticleLanding/2017/BM/C6BM00954A
Colecciones
Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Articulos(INQUISUR)
Articulos de INST.DE QUIMICA DEL SUR
Articulos(CCT - PATAGONIA NORTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA NORTE
Articulos(INBIOSUR)
Articulos de INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Citación
Agotegaray, Mariela Alejandra; Campelo, Adrián Esteban; Zysler, Roberto Daniel; Gumilar, Fernanda Andrea; Bras, Cristina Liliana; et al.; Magnetic nanoparticles for drug targeting: From design to insights into systemic toxicity. Preclinical evaluation of hematological, vascular and neurobehavioral toxicology; Royal Society of Chemistry; Biomaterials Science; 5; 4; 4-2017; 772-783
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