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dc.contributor.author
Suares, Alejandra Carolina
dc.contributor.author
Russo, Ana Josefa
dc.contributor.author
Verstuyf, Annemieke
dc.contributor.author
Boland, Ricardo Leopoldo
dc.contributor.author
González Pardo, María Verónica
dc.date.available
2016-07-04T19:10:53Z
dc.date.issued
2015-08
dc.identifier.citation
Suares, Alejandra Carolina; Russo, Ana Josefa; Verstuyf, Annemieke ; Boland, Ricardo Leopoldo; González Pardo, María Verónica; The proapoptotic protein Bim is up regulated by 1α,25-dihydroxyvitamin D3 and its receptor agonist in endothelial cells and transformed by viral GPCR associated to Kaposi sarcoma; Elsevier Science Inc; Steroids; 102; 8-2015; 85-91
dc.identifier.issn
0039-128X
dc.identifier.uri
http://hdl.handle.net/11336/6334
dc.description.abstract
We have previously shown that 1a,25-dihydroxyvitamin D3 [1a,25(OH)2D3] and its less calcemic analogTX 527 induce apoptosis via caspase-3 activation in endothelial cells (SVEC) and endothelial cells transformedby the viral G protein-coupled receptor associated to Kaposi sarcoma (vGPCR). In this work, westudied whether intrinsic apoptotic pathway could be activated by changing the balance between antiand pro-apoptotic proteins. Time response qRT-PCR analysis demonstrated that the mRNA level ofanti-apoptotic gene Bcl-2 decreased after 12 h and increased after 48 h treatment with 1a,25(OH)2D3or TX 527 in SVEC and vGPCR cells, whereas its protein level remained unchanged through time.mRNA levels of pro-apoptotic gene Bax significantly increased only in SVEC after 24 and 48 h treatmentwith 1a,25(OH)2D3 and TX 527 although its protein levels remained unchanged in both cell lines. BimmRNA and protein levels increased in SVEC and vGPCR cells. Bim protein increase by 1a,25(OH)2D3and TX 527 was abolished when the expression of vitamin D receptor (VDR) was suppressed. On the otherhand, Bortezomib (0.25?1 nM), an inhibitor of NF-jB pathway highly activated in vGPCR cells, increasedBim protein levels and induced caspase-3 cleavage. Altogether, these results indicate that 1a,25(OH)2D3and TX 527 trigger apoptosis by Bim protein increase which turns into the activation of caspase-3 in SVECand vGPCR cells. Moreover, this effect is mediated by VDR and involves NF-jB pathway inhibition invGPCR.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Vitamin D
dc.subject
Apoptosis
dc.subject
Kaposi Sarcoma
dc.subject
Endothelial Cells
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
The proapoptotic protein Bim is up regulated by 1α,25-dihydroxyvitamin D3 and its receptor agonist in endothelial cells and transformed by viral GPCR associated to Kaposi sarcoma
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-10T14:38:42Z
dc.journal.volume
102
dc.journal.pagination
85-91
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Suares, Alejandra Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: Verstuyf, Annemieke. Katholikie Universiteit Leuven; Bélgica
dc.description.fil
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.journal.title
Steroids
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.steroids.2015.08.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039128X15002196
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