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dc.contributor.author
Plácido, Alexandra
dc.contributor.author
Coelho, Andreia
dc.contributor.author
Nascimento, Lucas Abreu
dc.contributor.author
Vasconcelos, Andreanne Gomes
dc.contributor.author
Barroso, María Fátima
dc.contributor.author
Ramos Jesús, Joilson
dc.contributor.author
Costa, Vladimir
dc.contributor.author
Lima, Francisco das Chagas Alves
dc.contributor.author
Delerue Matos, Cristina
dc.contributor.author
Ramos, Ricardo Martins
dc.contributor.author
Marani, Mariela Mirta
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dc.contributor.author
Leite, José Roberto de Souza Almeida
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dc.date.available
2018-10-30T19:06:27Z
dc.date.issued
2017-07
dc.identifier.citation
Plácido, Alexandra; Coelho, Andreia; Nascimento, Lucas Abreu; Vasconcelos, Andreanne Gomes; Barroso, María Fátima; et al.; Cry1A(b)16 toxin from Bacillus thuringiensis: Theoretical refinement of three-dimensional structure and prediction of peptides as molecular markers for detection of genetically modified organisms; Wiley-liss, Div John Wiley & Sons Inc; Proteins: Structure, Function And Genetics; 85; 7; 7-2017; 1248-1257
dc.identifier.uri
http://hdl.handle.net/11336/63337
dc.description.abstract
Transgenic maize produced by the insertion of the Cry transgene into its genome became the second most cultivated crop worldwide. Cry gene from Bacillus thuringiensis kurstaki expresses protein derivatives of crystalline endotoxins which confer insect resistance onto the maize crop. Mandatory labeling of processed food containing or made by genetically modified organisms is in force in many countries, so, it is very urgent to develop fast and practical methods for GMO identification, for example, biosensors. In the absence of an available empirical structure of Cry1A(b)16 protein, a theoretical model was effectively generated, in this work, by homology modeling and molecular dynamics simulations based on two available homologous protein structures. Molecular dynamics simulations were carried out to refine the selected model, and an analysis of its global structure was performed. The refined models of Cry1A(b)16 showed a standard fold and structural characteristics similar to those seen in Bacillus thuringiensis Cry1A(a) insecticidal toxin and Bacillus thuringiensis serovar kurstaki Cry1A(c) toxin. After in silico analysis of Cry1A(b)16, two immunoreactive candidate peptides were selected and specific polyclonal antibodies were produced resulting in antibody–peptide interaction. Biosensing devices are expected to be developed for detection of the Cry1A(b) protein as a marker of transgenic maize in food. Proteins 2017; 85:1248–1257.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bacillus Thuringiensis
dc.subject
Cry1a(B)16
dc.subject
Homology Modeling
dc.subject
Molecular Dynamics Simulation
dc.subject.classification
Ciencias de la Computación
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dc.subject.classification
Ciencias de la Computación e Información
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Cry1A(b)16 toxin from Bacillus thuringiensis: Theoretical refinement of three-dimensional structure and prediction of peptides as molecular markers for detection of genetically modified organisms
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-23T13:31:12Z
dc.identifier.eissn
1097-0134
dc.journal.volume
85
dc.journal.number
7
dc.journal.pagination
1248-1257
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Plácido, Alexandra. Instituto Politécnico do Porto; Portugal
dc.description.fil
Fil: Coelho, Andreia. Instituto Politécnico do Porto; Portugal
dc.description.fil
Fil: Nascimento, Lucas Abreu. Universidade Federal do Piauí; Brasil
dc.description.fil
Fil: Vasconcelos, Andreanne Gomes. Universidade Federal do Piauí; Brasil
dc.description.fil
Fil: Barroso, María Fátima. Instituto Politécnico do Porto; Portugal
dc.description.fil
Fil: Ramos Jesús, Joilson. Universidade Federal do Piauí; Brasil
dc.description.fil
Fil: Costa, Vladimir. Instituto de Doenças Tropicais Natan Portela; Brasil
dc.description.fil
Fil: Lima, Francisco das Chagas Alves. Universidade Estadual do Piauí; Brasil
dc.description.fil
Fil: Delerue Matos, Cristina. Instituto Politécnico do Porto; Portugal
dc.description.fil
Fil: Ramos, Ricardo Martins. Universidade Estadual do Piauí; Brasil. Instituto Federal do Piauí; Brasil
dc.description.fil
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
dc.description.fil
Fil: Leite, José Roberto de Souza Almeida. Universidade de Brasília; Brasil
dc.journal.title
Proteins: Structure, Function And Genetics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/prot.25285/epdf
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/prot.25285
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