Artículo
Oncolytic Adenovirus-Loaded Menstrual Blood Stem Cells Overcome the Blockade of Viral Activity Exerted by Ovarian Cancer Ascites
Alfano, Ana Laura
; Nicola Candia, Alejandro Javier; Cuneo, Nicasio; Guttlein, Leandro N.; Soderini, Alejandro; Rotondaro, Cecilia
; Sganga, Leonardo
; Podhajcer, Osvaldo Luis
; Lopez, Maria Veronica
Fecha de publicación:
09/2017
Editorial:
American Society of Gene and Cell Therapy
Revista:
Molecular Therapy - Oncolytics
ISSN:
2372-7705
e-ISSN:
2372-7705
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Patients with ovarian cancer present peritoneal ascites at recurrence as a marker of disseminated disease and dismal prognosis. Oncolytic immunotherapy is an emerging approach for the treatment of disseminated cancer. In the present work, we constructed a novel oncolytic adenovirus, AR2011, to target malignant ovarian tumors. AR2011 exhibited a clear lytic effect in vitro in human ovarian cancer cell lines and malignant cells obtained from ascitic fluids (AFs) of patients with ovarian cancer. AR2011 activity was neutralized by antibodies present in 31 samples of patient-derived AFs. However, this blockade was overridden by preloading menstrual blood stem cells (MenSCs) with AR2011 (MenSC-AR), since AFs exerted no in vitro inhibitory effect on viral lytic activity under these conditions. Moreover, soluble factors present in AFs act as MenSC chemoattractants. MenSC-AR treatment of nude mice carrying established peritoneal carcinomatosis following administration of human ovarian cancer cells was able to inhibit tumor growth at levels similar to those observed with AR2011 alone. This study demonstrates that MenSCs can be used to override the blockade that AFs exert on viral oncolytic effects.
Palabras clave:
Crad
,
Msc
,
Ovarian Cancer
,
Peritoneal Carcinosis
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Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Alfano, Ana Laura; Nicola Candia, Alejandro Javier; Cuneo, Nicasio; Guttlein, Leandro N.; Soderini, Alejandro; et al.; Oncolytic Adenovirus-Loaded Menstrual Blood Stem Cells Overcome the Blockade of Viral Activity Exerted by Ovarian Cancer Ascites; American Society of Gene and Cell Therapy; Molecular Therapy - Oncolytics; 6; 9-2017; 31-44
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