Show simple item record

dc.contributor.author Guzman, Maria Laura
dc.contributor.author Marques, Margareth R.
dc.contributor.author Olivera, Maria Eugenia
dc.contributor.author Stippler, Erika S.
dc.date.available 2018-10-24T16:47:42Z
dc.date.issued 2016-03
dc.identifier.citation Guzman, Maria Laura; Marques, Margareth R.; Olivera, Maria Eugenia; Stippler, Erika S.; Enzymatic activity in the presence of surfactants commonly used in dissolution media, Part 1: Pepsin; Elsevier; Results in Pharma Sciences; 6; 3-2016; 15-19
dc.identifier.issn 2211-2863
dc.identifier.uri http://hdl.handle.net/11336/62988
dc.description.abstract The United States Pharmacopeia (USP) General Chapters Dissolution 〈711〉 and Disintegration and Dissolution of Dietary Supplements 〈2040〉 allows the use of enzymes in dissolution media when gelatin capsules do not conform to dissolution specifications due to cross linking. Possible interactions between enzymes and surfactants when used together in dissolution media could result in loss of the enzymatic activity. Pepsin is an enzyme commonly used in dissolution media, and in this work, the activity of pepsin was determined in the presence of different surfactants as usually found in case of dissolution tests of certain gelatin capsule formulations.Pepsin enzymatic activity was determined according to the Ninth Edition of the Food Chemicals Codex (FCC) 9 method, in dissolution conditions: simulated gastric fluid, 37 °C and 50 rpm. Sodium dodecyl sulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), polysorbate 80 (Tween 80) and octoxynol 9 (Triton X100) in concentrations above and below their critical micellar concentrations were selected. Results showed a significant reduction in the activity of pepsin at all the concentrations of SDS assayed. On the contrary, CTAB, Tween 80, and Triton X100 did not alter the enzymatic activity at of pepsin any of the concentration assayed.This data demonstrates a rational selection of the surfactant to be used when pepsin is required in dissolution test.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject DISSOLUTION TEST
dc.subject ENZYMES
dc.subject PEPSIN
dc.subject SURFACTANTS
dc.subject.classification Otras Ciencias de la Salud
dc.subject.classification Ciencias de la Salud
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Enzymatic activity in the presence of surfactants commonly used in dissolution media, Part 1: Pepsin
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-10-23T13:44:55Z
dc.journal.volume 6
dc.journal.pagination 15-19
dc.journal.pais Países Bajos
dc.description.fil Fil: Guzman, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
dc.description.fil Fil: Marques, Margareth R.. Pharmacopeia; Estados Unidos
dc.description.fil Fil: Olivera, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
dc.description.fil Fil: Stippler, Erika S.. Pharmacopeia; Estados Unidos
dc.journal.title Results in Pharma Sciences
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.rinphs.2016.02.002
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211286316300021
dc.conicet.fuente individual


Archivos asociados

This item appears in the following Collection(s)

  • Articulos(UNITEFA) [130]
    Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO EN TECNOLOGIA FARMACEUTICA

Show simple item record

info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)