Artículo
Preliminary characterization of dexamethasone-loaded cross-linked hyaluronic acid films for topical ocular therapy
Calles, Javier Adrián
; López García, A.; Valles, Enrique Marcelo
; Palma, Santiago Daniel
; Diebold Luque, Yolanda
Fecha de publicación:
07/2016
Editorial:
Elsevier Science
Revista:
International Journal Of Pharmaceutics
ISSN:
0378-5173
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this work was to design and characterize cross-linked hyaluronic acid (HA)-itaconic acid (IT) films loaded with dexamethasone sodium phosphate salt (DEX) for topical therapy of inflammatory ocular surface diseases. Films were chemically cross-linked with polyethylene glycol diglycidyl ether (PEGDE), then physical and mechanical characterization by stress-strain, X-ray diffraction, X-ray fluorescence spectrometry and swelling assays was conducted. A sequential in vitro therapeutic efficacy model was designed to assess changes in interleukin (IL)-6 production in an inflamed human corneal epithelial (HCE) cell line after film exposure. Changes in cell proliferation after film exposure were assessed using the alamarBlue® proliferation assay. Experimental findings showed desirable mechanical properties and in vitro efficacy to reduce cell inflammation. A moderately decreased proliferation rate was induced in HCE cells by DEX-loaded films, compared to commercial DEX eye drops. These results suggest that DEX and HA have opposite effects. The sequential in vitro therapeutic efficacy model arises as an efficient tool to study drug release from delivery systems by indirect measurement of a biological response.
Palabras clave:
Dexamethasone
,
Hyaluronan
,
Inflammation
,
Model
,
Ocular
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Identificadores
Colecciones
Articulos(PLAPIQUI)
Articulos de PLANTA PILOTO DE INGENIERIA QUIMICA (I)
Articulos de PLANTA PILOTO DE INGENIERIA QUIMICA (I)
Articulos(UNITEFA)
Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO EN TECNOLOGIA FARMACEUTICA
Articulos de UNIDAD DE INVESTIGACION Y DESARROLLO EN TECNOLOGIA FARMACEUTICA
Citación
Calles, Javier Adrián; López García, A.; Valles, Enrique Marcelo; Palma, Santiago Daniel; Diebold Luque, Yolanda; Preliminary characterization of dexamethasone-loaded cross-linked hyaluronic acid films for topical ocular therapy; Elsevier Science; International Journal Of Pharmaceutics; 509; 1-2; 7-2016; 237-243
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