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dc.contributor.author
Martin, Sophie
dc.contributor.author
Bellora, Nicolás
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González Vallinas, Juan
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Irimia, Manuel
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Chebli, Karim
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de Toledo, Marion
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Raabe, Monika
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Eyras, Eduardo
dc.contributor.author
Urlaub, Henning
dc.contributor.author
Blencowe, Ben J.
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Tazi, Jamal
dc.date.available
2018-10-23T21:13:43Z
dc.date.issued
2016-11
dc.identifier.citation
Martin, Sophie; Bellora, Nicolás; González Vallinas, Juan; Irimia, Manuel; Chebli, Karim; et al.; Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 139; 3; 11-2016; 349-368
dc.identifier.issn
0022-3042
dc.identifier.uri
http://hdl.handle.net/11336/62969
dc.description.abstract
Neuronal granules play an important role in the localization and transport of translationally silenced messenger ribonucleoproteins in neurons. Among the factors associated with these granules, the RNA-binding protein G3BP1 (stress-granules assembly factor) is involved in neuronal plasticity and is induced in Alzheimer's disease. We immunopurified a stable complex containing G3BP1 from mouse brain and performed high-throughput sequencing and cross-linking immunoprecipitation to identify the associated RNAs. The G3BP-complex contained the deubiquitinating protease USP10, CtBP1 and the RNA-binding proteins Caprin-1, G3BP2a and splicing factor proline and glutamine rich, or PSF. The G3BP-complex binds preferentially to transcripts that retain introns, and to non-coding sequences like 3’-untranslated region and long non-coding RNAs. Specific transcripts with retained introns appear to be enriched in the cerebellum compared to the rest of the brain and G3BP1 depletion decreased this intron retention in the cerebellum of G3BP1 knockout mice. Among the enriched transcripts, we found an overrepresentation of genes involved in synaptic transmission, especially glutamate-related neuronal transmission. Notably, G3BP1 seems to repress the expression of the mature Grm5 (metabotropic glutamate receptor 5) transcript, by promoting the retention of an intron in the immature transcript in the cerebellum. Our results suggest that G3BP is involved in a new functional mechanism to regulate non-coding RNAs including intron-retaining transcripts, and thus have broad implications for neuronal gene regulation, where intron retention is widespread.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cerebellum
dc.subject
G3bp
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Hits-Clip
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Retained Intron
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Stress Granules
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-22T13:17:45Z
dc.journal.volume
139
dc.journal.number
3
dc.journal.pagination
349-368
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Martin, Sophie. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia
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Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
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Fil: González Vallinas, Juan. Universitat Pompeu Fabra; España
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Fil: Irimia, Manuel. University of Toronto; Canadá
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Fil: Chebli, Karim. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia
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Fil: de Toledo, Marion. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia
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Fil: Raabe, Monika. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Eyras, Eduardo. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Pompeu Fabra; España
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Fil: Urlaub, Henning. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Blencowe, Ben J.. University of Toronto; Canadá
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Fil: Tazi, Jamal. Institut de Génétique Moléculaire de Montpellier; Fiyi. Centre National de la Recherche Scientifique; Francia
dc.journal.title
Journal of Neurochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jnc.13768/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/jnc.13768
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