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dc.contributor.author Fernández, Gabriela Cristina
dc.contributor.author Ramos, Maria Victoria
dc.contributor.author Gómez, Sonia Alejandra
dc.contributor.author Dran, Graciela Isabel
dc.contributor.author Exeni, Ramón
dc.contributor.author Alduncin, Marta
dc.contributor.author Grimoldi, Irene
dc.contributor.author Vallejo, Graciela
dc.contributor.author Elias Costa, Christian
dc.contributor.author Isturiz, Martín Amadeo
dc.contributor.author Palermo, Marina Sandra
dc.date.available 2018-10-22T20:16:48Z
dc.date.issued 2005-10
dc.identifier.citation Fernández, Gabriela Cristina; Ramos, Maria Victoria; Gómez, Sonia Alejandra; Dran, Graciela Isabel; Exeni, Ramón; et al.; Differential expression of function-related antigens on blood monocytes in children with hemolytic uremic syndrome; Federation of American Societies for Experimental Biology; Journal of Leukocyte Biology; 78; 4; 10-2005; 853-861
dc.identifier.issn 0741-5400
dc.identifier.uri http://hdl.handle.net/11336/62877
dc.description.abstract Monocytes (Mo) mediate central functions in inflammation and immunity. Different subpopulations of Mo with distinct phenotype and functional properties have been described. Here, we investigate the phenotype and function of peripheral Mo from children with hemolytic uremic syndrome (HUS). For this purpose, blood samples from patients m the acute period of HUS (HUS AP) were obtained on admission before dialysis and/or transfusion. The Mo phenotypic characterization was performed on whole blood by flow cytometry, and markers associated to biological functions were selected: CD14 accounting for lipopolysaccharide (LPS) responsiveness, CD11b for adhesion, Fc receptor for mimunoglobulin G type I (FcγRI)/CD64 for phagocytosis and cytotoxicity, and human leukocyte antigen (HLA)-DR for antigen presentation. Some of these functions were also determined. Moreover, the percentage of CD14+ CD16+ Mo was evaluated. We found that the entire HUS AP Mo population exhibited reduced CD14, CD64, and CD11b expression and decreased LPS-mduced tumor necrosis factor production and Fcγ-dependent cytotoxicity. HUS AP showed an increased percentage of CD14+ CD16+ Mo with higher CD16 and lower CD14 levels compared with the same subset from healthy children. Moreover, the CD14++ CD16- Mo sub-population of HUS AP had a decreased HLA-DR expression, which correlated with severity. In conclusion, the Mo population from HUS AP patients presents phenotypic and functional alterations. The contribution to the pathogenesis and the possible scenarios that led to these changes are discussed.
dc.format application/pdf
dc.language.iso eng
dc.publisher Federation of American Societies for Experimental Biology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CD14
dc.subject CD16
dc.subject HLA-DR
dc.subject HUS
dc.subject TNF
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Differential expression of function-related antigens on blood monocytes in children with hemolytic uremic syndrome
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-10-12T17:27:53Z
dc.journal.volume 78
dc.journal.number 4
dc.journal.pagination 853-861
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.description.fil Fil: Fernández, Gabriela Cristina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Ramos, Maria Victoria. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Gómez, Sonia Alejandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil Fil: Exeni, Ramón. Municipio de La Matanza. Hospital Pediátrico Municipal de San Justo; Argentina
dc.description.fil Fil: Alduncin, Marta. Municipio de La Matanza. Hospital Pediátrico Municipal de San Justo; Argentina
dc.description.fil Fil: Grimoldi, Irene. Municipio de La Matanza. Hospital Pediátrico Municipal de San Justo; Argentina
dc.description.fil Fil: Vallejo, Graciela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil Fil: Elias Costa, Christian. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil Fil: Isturiz, Martín Amadeo. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Palermo, Marina Sandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Journal of Leukocyte Biology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1189/jlb.0505251
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://jlb.onlinelibrary.wiley.com/doi/abs/10.1189/jlb.0505251
dc.conicet.fuente individual


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)