Mostrar el registro sencillo del ítem

dc.contributor.author
Fernández, Gabriela Cristina
dc.contributor.author
Ramos, Maria Victoria
dc.contributor.author
Gómez, Sonia Alejandra
dc.contributor.author
Dran, Graciela Isabel
dc.contributor.author
Exeni, Ramón
dc.contributor.author
Alduncin, Marta
dc.contributor.author
Grimoldi, Irene
dc.contributor.author
Vallejo, Graciela
dc.contributor.author
Elias Costa, Christian
dc.contributor.author
Isturiz, Martín Amadeo
dc.contributor.author
Palermo, Marina Sandra
dc.date.available
2018-10-22T20:16:48Z
dc.date.issued
2005-10
dc.identifier.citation
Fernández, Gabriela Cristina; Ramos, Maria Victoria; Gómez, Sonia Alejandra; Dran, Graciela Isabel; Exeni, Ramón; et al.; Differential expression of function-related antigens on blood monocytes in children with hemolytic uremic syndrome; Federation of American Societies for Experimental Biology; Journal of Leukocyte Biology; 78; 4; 10-2005; 853-861
dc.identifier.issn
0741-5400
dc.identifier.uri
http://hdl.handle.net/11336/62877
dc.description.abstract
Monocytes (Mo) mediate central functions in inflammation and immunity. Different subpopulations of Mo with distinct phenotype and functional properties have been described. Here, we investigate the phenotype and function of peripheral Mo from children with hemolytic uremic syndrome (HUS). For this purpose, blood samples from patients m the acute period of HUS (HUS AP) were obtained on admission before dialysis and/or transfusion. The Mo phenotypic characterization was performed on whole blood by flow cytometry, and markers associated to biological functions were selected: CD14 accounting for lipopolysaccharide (LPS) responsiveness, CD11b for adhesion, Fc receptor for mimunoglobulin G type I (FcγRI)/CD64 for phagocytosis and cytotoxicity, and human leukocyte antigen (HLA)-DR for antigen presentation. Some of these functions were also determined. Moreover, the percentage of CD14+ CD16+ Mo was evaluated. We found that the entire HUS AP Mo population exhibited reduced CD14, CD64, and CD11b expression and decreased LPS-mduced tumor necrosis factor production and Fcγ-dependent cytotoxicity. HUS AP showed an increased percentage of CD14+ CD16+ Mo with higher CD16 and lower CD14 levels compared with the same subset from healthy children. Moreover, the CD14++ CD16- Mo sub-population of HUS AP had a decreased HLA-DR expression, which correlated with severity. In conclusion, the Mo population from HUS AP patients presents phenotypic and functional alterations. The contribution to the pathogenesis and the possible scenarios that led to these changes are discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Federation of American Societies for Experimental Biology
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cd14
dc.subject
Cd16
dc.subject
Hla-Dr
dc.subject
Hus
dc.subject
Tnf
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Differential expression of function-related antigens on blood monocytes in children with hemolytic uremic syndrome
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-12T17:27:53Z
dc.journal.volume
78
dc.journal.number
4
dc.journal.pagination
853-861
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Fernández, Gabriela Cristina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ramos, Maria Victoria. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gómez, Sonia Alejandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Exeni, Ramón. Municipio de La Matanza. Hospital Pediátrico Municipal de San Justo; Argentina
dc.description.fil
Fil: Alduncin, Marta. Municipio de La Matanza. Hospital Pediátrico Municipal de San Justo; Argentina
dc.description.fil
Fil: Grimoldi, Irene. Municipio de La Matanza. Hospital Pediátrico Municipal de San Justo; Argentina
dc.description.fil
Fil: Vallejo, Graciela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Elias Costa, Christian. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Isturiz, Martín Amadeo. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Palermo, Marina Sandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Leukocyte Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1189/jlb.0505251
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://jlb.onlinelibrary.wiley.com/doi/abs/10.1189/jlb.0505251


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem