Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)

Abstract

The freshwater snail Galba cubensis (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of Fasciola hepatica - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in G. cubensis to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of G. cubensis from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: G. truncatula, G. viator and G. neotropica. We found that G. cubensis has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in G. truncatula and G. viator, respectively, and that all 15 loci amplified in G. neotropica. This last finding suggests a close relatedness between G. cubensis and G. neotropica, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.