Artículo
Neuronal loss and abnormal BMP/Smad signaling in the myenteric plexus of diabetic rats
Honore, Stella Maris
; Zelarayán, Laura C.; Genta, Susana Beatriz de Fátima; Sanchez, Sara Serafina del V.
Fecha de publicación:
10/2011
Editorial:
Elsevier Science
Revista:
Autonomic Neuroscience
ISSN:
1566-0702
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Bone morphogenetic proteins (BMPs) are critical molecules during gut morphogenesis. However, little is known about their participation in the homeostasis of adult gut and their possible role in diseases. Gastrointestinal complications occur during diabetes with loss of enteric neurons. In this study, we investigated the possible involvement of BMPs signaling pathway in diabetic enteric neuropathy in an experimental model of diabetes in rats. The expression of BMPs, BMPs receptors and intracellular Smad effectors were assessed in control and diabetic smooth muscle layer of jejunum by immunofluorescence, Western blot and RT-PCR methods. Myenteric neurons and glial cells were measured by immunofluorescence using specific markers. In addition, cell apoptosis was evaluated by means of direct and indirect techniques. We demonstrated that diabetic ganglia displayed a significant decrease in ganglion size due to enhanced apoptosis and loss of peripherin. A decrease in glial fibrillary acidic protein (GFAP protein) was also observed in enteric glial cells. BMP-2 was down-regulated in the myenteric plexus of diabetic rats at 3 and 9. weeks. A loss of enteric neurons by apoptosis was correlated with an ectopic BMP-4, increased BMPR-Ia and nuclear p-Smad1 expression in the myenteric plexus. Insulin-treatment prevented the intestinal alterations observed. These findings suggest that diabetes is associated with an abnormal BMP/Smad signaling expression in the myenteric ganglia that affects the homeostasis of the enteric plexus.
Palabras clave:
Bmp/Smad Signaling
,
Diabetes
,
Enteric Nervous System
,
Small Intestine
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Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Honore, Stella Maris; Zelarayán, Laura C.; Genta, Susana Beatriz de Fátima; Sanchez, Sara Serafina del V.; Neuronal loss and abnormal BMP/Smad signaling in the myenteric plexus of diabetic rats; Elsevier Science; Autonomic Neuroscience; 164; 1-2; 10-2011; 51-61
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