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dc.contributor.author
Hauk, Pricila
dc.contributor.author
Negrotto, Soledad
dc.contributor.author
Romero, Eliete Caló
dc.contributor.author
Vasconcellos, Sílvio Arruda
dc.contributor.author
Genovez, Margareth Élide
dc.contributor.author
Ward, Richard John
dc.contributor.author
Schattner, Mirta Ana
dc.contributor.author
Gomez, Ricardo Martin
dc.contributor.author
Ho, Paulo Lee
dc.date.available
2018-10-18T13:59:01Z
dc.date.issued
2005-08
dc.identifier.citation
Hauk, Pricila; Negrotto, Soledad; Romero, Eliete Caló; Vasconcellos, Sílvio Arruda; Genovez, Margareth Élide; et al.; Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 333; 4; 8-2005; 1341-1347
dc.identifier.issn
0006-291X
dc.identifier.uri
http://hdl.handle.net/11336/62676
dc.description.abstract
The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection. © 2005 Elsevier Inc. All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hemolysin
dc.subject
Hlyx
dc.subject
Leptospira Interrogans Serovar Copenhageni
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Leptospirosis
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Lipl32
dc.subject.classification
Medicina Critica y de Emergencia
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Medicina Clínica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-12T17:27:48Z
dc.journal.volume
333
dc.journal.number
4
dc.journal.pagination
1341-1347
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Hauk, Pricila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Negrotto, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Romero, Eliete Caló. Instituto Adolfo Lutz; Brasil
dc.description.fil
Fil: Vasconcellos, Sílvio Arruda. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Genovez, Margareth Élide. Centro de Pesquisa e Desenvolvimento de Sanidade Animal; Brasil
dc.description.fil
Fil: Ward, Richard John. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.description.fil
Fil: Ho, Paulo Lee. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
dc.journal.title
Biochemical and Biophysical Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbrc.2005.06.045
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0006291X05012623
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