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dc.contributor.author
González Pardo, María Verónica
dc.contributor.author
D'elía, Noelia Laura
dc.contributor.author
Berstuy, Annemieke
dc.contributor.author
Boland, Ricardo Leopoldo
dc.contributor.author
Russo, Ana Josefa
dc.date.available
2018-10-17T15:41:55Z
dc.date.issued
2012-05
dc.identifier.citation
González Pardo, María Verónica; D'elía, Noelia Laura; Berstuy, Annemieke; Boland, Ricardo Leopoldo; Russo, Ana Josefa; The NFkB pathway is down-regulated by 1α,25(OH)2-Vitamin D3 in endothelial cells transformed by Kaposi Sarcoma-associated herpes virus G protein coupled receptor; Elsevier Science Inc; Steroids; 77; 5-2012; 1025-1032
dc.identifier.issn
0039-128X
dc.identifier.uri
http://hdl.handle.net/11336/62541
dc.description.abstract
We have previously demonstrated that 1α,25 dihydroxy-vitamin D3 (1α,25(OH)2D3) has antiproliferative effects on the growth of endothelial cells transformed by the viral G protein-coupled receptor associated to Kaposi sarcoma (vGPCR). In this work, we have investigated whether 1α,25(OH)2D3 exerts its growth inhibitory effects by inhibiting the Nuclear Factor κ B (NFκB) pathway which is highly activated by vGPCR. Cell proliferation studies demonstrated that 1α,25(OH)2D3, similarly to bortezomib, a proteosome inhibitor that suppresses the activation of NFκB, reduced the proliferation of endothelial cells transformed by vGPCR (SVEC-vGPCR). The activity of NFκB in these cells decreased by 70% upon 1α,25(OH)2D3 treatment. Furthermore, time and dose response studies showed that the hormone significantly decreased NFκB and increased IκBα mRNA and protein levels in SVEC-vGPCR cells, whereas in SVEC only IκBα increased significantly. Moreover, NFκB translocation to the nucleus was inhibited and occurred by a mechanism independent of NFκB association with vitamin D3 receptor (VDR). 1α,25(OH)2D3-induced increase in IκBα required de novo protein synthesis, and was independent of MAPK and PI3K/Akt pathways. Altogether, these results suggest that down-regulation of the NFκB pathway is part of the mechanism involved in the antiproliferative effects of 1α,25(OH)2D3 on endothelial cells transformed by vGPCR.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Nfkb
dc.subject
1α,25(Oh)2-Vitamin D3
dc.subject
Endothelial Cells
dc.subject
Kaposi Sarcoma
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
The NFkB pathway is down-regulated by 1α,25(OH)2-Vitamin D3 in endothelial cells transformed by Kaposi Sarcoma-associated herpes virus G protein coupled receptor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-12T14:45:45Z
dc.journal.volume
77
dc.journal.pagination
1025-1032
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Katholieke Universiteit Leuven; Bélgica
dc.description.fil
Fil: D'elía, Noelia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Berstuy, Annemieke. Katholieke Universiteit Leuven; Bélgica
dc.description.fil
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.journal.title
Steroids
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039128X12001808
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.steroids.2012.05.006
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