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dc.contributor.author
Siena, Lorena Adelina
dc.contributor.author
Sartor, Maria Esperanza
dc.contributor.author
Quarin, Camilo Luis
dc.contributor.author
Espinoza, Francisco
dc.contributor.author
Ortiz, Juan Pablo Amelio
dc.date.available
2016-06-16T14:03:36Z
dc.date.issued
2013-11
dc.identifier.citation
Siena, Lorena Adelina; Sartor, Maria Esperanza; Quarin, Camilo Luis; Espinoza, Francisco; Ortiz, Juan Pablo Amelio; Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping; Crop Science Society of America; Crop Science; 54; 1; 11-2013; 240-254
dc.identifier.issn
0011-183X
dc.identifier.uri
http://hdl.handle.net/11336/6222
dc.description.abstract
The genus Paspalum includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat EST-SSR (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum genomic-SSRs (PnSSR) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty two accessions, including 11 species were used. Moreover, 65 F1 hybrids of P. notatum were employed for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSRs data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both, wEST-SSRs and PnSSRs mapped scattered in the genome. On hundred and two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219 and PN03-F2 generated markers that mapped linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23% to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Crop Science Society of America
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Paspalum Spp
dc.subject
Wheat Est-Ssr
dc.subject
Apospory
dc.subject
Comparative Mapping
dc.subject.classification
Agronomía, reproducción y protección de plantas
dc.subject.classification
Agricultura, Silvicultura y Pesca
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Transference of wheat Expressed Sequence Tag-Simple Sequence Repits to Paspalum species and cross-species amplification of Paspalum notatum Simple Sequence Repits: Potential use in phylogenetic analysis and mapping
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-01-05T16:31:34Z
dc.journal.volume
54
dc.journal.number
1
dc.journal.pagination
240-254
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Baltimore
dc.description.fil
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
dc.description.fil
Fil: Sartor, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
dc.description.fil
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
dc.description.fil
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
dc.description.fil
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
dc.journal.title
Crop Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2013.04.0275
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/54/1/240
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2135/cropsci2013.04.0275


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