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dc.contributor.author
Gutiérrez Cacciabue, Dolores  
dc.contributor.author
Cid, Alicia Graciela  
dc.contributor.author
Rajal, Verónica Beatriz  
dc.date.available
2018-10-10T20:44:01Z  
dc.date.issued
2016-01  
dc.identifier.citation
Gutiérrez Cacciabue, Dolores; Cid, Alicia Graciela; Rajal, Verónica Beatriz; How long can culturable bacteria and total DNA persist in environmental waters? The role of sunlight and solid particles; Elsevier Science; Science of the Total Environment; 539; 1-2016; 494-502  
dc.identifier.issn
0048-9697  
dc.identifier.uri
http://hdl.handle.net/11336/62151  
dc.description.abstract
In this work, sunlight inactivation of two indicator bacteria in freshwater, with and without solid particles, was studied and the persistence of culturable cells and total DNA was compared. Environmental water was used to prepare two matrices, with and without solid particles, which were spiked with Escherichia coli and Enterococcus faecalis. These matrices were used to prepare microcosm bags that were placed in two containers: one exposed to sunlight and the other in the dark. During one month, samples were removed from each container and detection was done by membrane filter technique and real-time PCR. Kinetic parameters were calculated to assess sunlight effect. Indicator bacteria without solid particles exposed to sunlight suffered an immediate decay (<4h) compared with the ones which were shielded from them. In addition, the survival of both bacteria with solid particles varied depending on the situation analyzed (T99 from 3 up to 60days), being always culturable E. coli more persistent than E. faecalis. On the other side, E. faecalis DNA persisted much longer than culturable cells (T99>40h in the dark with particles). In this case active cells were more prone to sunlight than total DNA and the protective effect of solid particles was also observed. Results highlight that the effects caused by the parameters which describe the behavior of culturable microorganisms and total DNA in water are different and must be included in simulation models but without forgetting that these parameters will also depend on bacterial properties, sensitizers, composition, type, and uses of the aquatic environment under assessment.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Bacterial Indicators  
dc.subject
Culture-Based Methods  
dc.subject
Environmental Waters  
dc.subject
Qpcr  
dc.subject
Solid Particles  
dc.subject
Sunlight Inactivation  
dc.subject.classification
Ingeniería Medioambiental y Geológica, Geotécnicas  
dc.subject.classification
Ingeniería del Medio Ambiente  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
How long can culturable bacteria and total DNA persist in environmental waters? The role of sunlight and solid particles  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-09-27T15:35:54Z  
dc.journal.volume
539  
dc.journal.pagination
494-502  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Gutiérrez Cacciabue, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Cid, Alicia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina. Nanyang Technological University; Singapur  
dc.journal.title
Science of the Total Environment  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.scitotenv.2015.07.138  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0048969715304800