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dc.contributor.author
Lassalle, Verónica Leticia
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dc.contributor.author
Ferreira, María Luján
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dc.date.available
2018-10-10T20:14:27Z
dc.date.issued
2009-12
dc.identifier.citation
Lassalle, Verónica Leticia; Ferreira, María Luján; Experimental problems in the application of UV/visible based methods as the quantification tool for the entrapped/released insulin from PLGA carriers; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 84; 9; 12-2009; 1263-1273
dc.identifier.issn
0268-2575
dc.identifier.uri
http://hdl.handle.net/11336/62134
dc.description.abstract
Background: Controlled release of medicaments from biodegradable polymers remains the most convenient way for their sustained release. Although a number of articles have been published, experimental work involving the preparation of polymer-based carriers and release procedures are not described with sufficient level of detail to allow other researchers to reproduce the experiments and to compare published results with their own. In this contribution the experimental background of the entrapment and release of insulin from PLGA carriers is described and the problems found at each step related to UV/Visible method used to quantify them are addressed in detail. Results: The quantification of entrapped insulin by UV/visible methods was affected by aggregation. The design of the release experiment influenced the results regarding the entrapment efficiency (EE) and the maximum percentage of released insulin. It was also found that the presence of colloidal polymeric particles, insufficient centrifugation times and the kind of solvent used in the release test might lead to mistakes in the percentage of liberated insulin when UV/visible based methods are employed. Conclusions: This contribution demonstrates that serious discrepancies in the EE and percentage of released protein may arise if some key experimental factors are not taken into account. Therefore, the analysis presented here tries to point out important aspects of this topic currently not reported, unnoticed or not properly analyzed in the open literature. The results are useful for the entrapment of any protein on any polymeric device using UV/visible based methods to quantify them. © 2009 Society of Chemical Industry.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
In Vitro Release
dc.subject
Insulin
dc.subject
Poly (Lactic-Co-Glycolic Acid)
dc.subject
Protein Entrapment
dc.subject
Sustained Release
dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Experimental problems in the application of UV/visible based methods as the quantification tool for the entrapped/released insulin from PLGA carriers
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-30T14:55:46Z
dc.journal.volume
84
dc.journal.number
9
dc.journal.pagination
1263-1273
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.description.fil
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
dc.journal.title
Journal of Chemical Technology and Biotechnology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/jctb.2171
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.2171
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