Artículo
Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
Pomares, Maria Fernanda
; Corbalan, Natalia Soledad
; Adler, Conrado
; de Cristobal, Ricardo Ezequiel
; Farias, Ricardo Norberto
; Delgado, Monica Alejandra
; Vincent, Paula Andrea
Fecha de publicación:
05/2013
Editorial:
Biomed Central Ltd
Revista:
Bmc Microbiology
ISSN:
1471-2180
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In this work we demonstrate that S. Typhimurium becomes notably susceptible to MccJ25 when replicating within macrophages. In order to determine the possible cause of this phenomenon, we studied the sensitivity of S. Typhimurium to MccJ25 at conditions resembling those of the internal macrophage environment, such as low pH, low magnesium and iron deprivation. We observed that the strain was only sensitive to the antibiotic at low pH, leading us to attribute the bacterial sensitization to this condition. A MccJ25-resistant E. coli strain in which fhuA is deleted was also inhibited by the antibiotic at low pH. Then, we could assume that the MccJ25 sensitivity change observed in both E. coli fhuA and S. Typhimurium is mediated by a MccJ25 uptake independent of the FhuA receptor. Moreover, low pH incubation also sensitized S. Typhimurium to the hydrophobic antibiotic novobiocin, which does not affect enteric bacteria viability because it is unable to penetrate the bacterial outer membrane. This observation supports our hypothesis about low pH producing a modification in the bacterial membrane permeability that allows an unspecific MccJ25 uptake. On the other hand, MccJ25 inhibited S. Typhimurium when cells were preincubated in acidic pH medium and then treated at neutral pH with the antibiotic.
Palabras clave:
Microcin
,
Salmonella
,
Target
,
Cell Line
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Pomares, Maria Fernanda; Corbalan, Natalia Soledad; Adler, Conrado; de Cristobal, Ricardo Ezequiel; Farias, Ricardo Norberto; Delgado, Monica Alejandra; Vincent, Paula Andrea; Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive; Biomed Central Ltd; Bmc Microbiology; 13; 5-2013; 1-6;
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