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dc.contributor.author
Alvarez, Florencia  
dc.contributor.author
Lozano Puerto, Rosa M.  
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Pérez-Maceda, Blanca  
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Grillo, Claudia Alejandra  
dc.contributor.author
Fernandez Lorenzo, Monica Alicia  
dc.date.available
2018-10-09T16:38:23Z  
dc.date.issued
2016-02  
dc.identifier.citation
Alvarez, Florencia; Lozano Puerto, Rosa M.; Pérez-Maceda, Blanca; Grillo, Claudia Alejandra; Fernandez Lorenzo, Monica Alicia; Time-Lapse Evaluation of Interactions between Biodegradable Mg Particles and Cells; Cambridge University Press; Microscopy & Microanalysis; 22; 1; 2-2016; 1-12  
dc.identifier.issn
1431-9276  
dc.identifier.uri
http://hdl.handle.net/11336/61974  
dc.description.abstract
Mg-based implants have promising applications as biodegradable materials in medicine for orthopedic, dental, and cardiovascular therapies. During wear and degradation microdebris are released. Time-lapse multidimensional microscopy (MM) is proposed here as a suitable tool to follow, in fixed intervals over 24-h periods, the interaction between cells and particles. Results of MM show interactions of macrophages (J774) with the magnesium particles (MgPa) that led to modifications of cell size and morphology, a decrease in duplication rate, and cell damage. Corrosion products were progressively formed on the surface of the particles and turbulence was generated due to hydrogen development. Changes were more significant after treating MgPa with potassium fluoride. In order to complement MM observations, membrane damage as detected by a lactase dehydrogenase (LDH) assay and mitochondrial activity as detected by a WST-1 assay with macrophages and osteoblasts (MC3T3-E1) were compared. A more significant concentration-dependent effect was detected for macrophages exposed to MgPa than for osteoblasts. Accordingly, complementary data showed that viability and cell cycle seem to be more altered in macrophages. In addition, protein profiles and expression of proteins associated with the adhesion process changed in the presence of MgPa. These studies revealed that time-lapse MM is a helpful tool for monitoring changes of biodegradable materials and the biological surrounding in real time and in situ. This information is useful in studies related to biodegradable biomaterials.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Cambridge University Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Biodegradation  
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Macrophage  
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Magnesium  
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Multidimensional Microscopy  
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Osteoblast  
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Biotecnología Industrial  
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Biotecnología Industrial  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Time-Lapse Evaluation of Interactions between Biodegradable Mg Particles and Cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-09-24T14:05:46Z  
dc.journal.volume
22  
dc.journal.number
1  
dc.journal.pagination
1-12  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Cambridge  
dc.description.fil
Fil: Alvarez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina  
dc.description.fil
Fil: Lozano Puerto, Rosa M.. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España  
dc.description.fil
Fil: Pérez-Maceda, Blanca. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España  
dc.description.fil
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina  
dc.description.fil
Fil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina  
dc.journal.title
Microscopy & Microanalysis  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1017/S1431927615015597  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/timelapse-evaluation-of-interactions-between-biodegradable-mg-particles-and-cells/98459528FD6C65E23A740D7554FD7315