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dc.contributor.author
González Pardo, María Verónica
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dc.contributor.author
Martin, Daniel
dc.contributor.author
Gutkind, J. Silvio
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dc.contributor.author
Verstuyf, Annemieke
dc.contributor.author
Bouillon, Roger
dc.contributor.author
Russo, Ana Josefa
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dc.contributor.author
Boland, Ricardo Leopoldo
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dc.date.available
2018-10-05T16:48:23Z
dc.date.issued
2010-01
dc.identifier.citation
González Pardo, María Verónica; Martin, Daniel; Gutkind, J. Silvio; Verstuyf, Annemieke; Bouillon, Roger; et al.; 1α,25-dihydroxyvitamin D3 and its TX527 analog inhibit the growth of endothelial cells transformed by Kaposi sarcoma-associated herpes virus G protein-coupled receptor in vitro and in vivo; Endocrine Society; Endocrinology; 151; 1; 1-2010; 23-31
dc.identifier.issn
0013-7227
dc.identifier.uri
http://hdl.handle.net/11336/61766
dc.description.abstract
The Kaposi sarcoma-associated herpes virus-G protein-coupled receptor is a key molecule in the pathogenesis of Kaposi sarcoma, playing a central role in promoting vascular endothelial growth factor-driven angiogenesis and spindle cell proliferation. We studied the effects of 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3] and the analog TX527 on the proliferation of endothelial cells (SVECs) and SVECs transformed by the viral G protein-coupled receptor (SVEC-vGPCR). 1α,25(OH)2D 3 and TX527 decreased SVEC-vGPCR and SVEC numbers, the response being time dependent and similar in both cell lines. Vitamin D receptor (VDR) levels increased on treatment with 10 nM 1α,25(OH)2D3 or 1 nM TX527 in a time-dependent manner (1.5-24 h) in SVECs and SVEC-vGPCR. Basal VDR levels were increased in SVEC-vGPCR. The antiproliferative effects were accompanied by reduction in cyclin D1 and accumulation of p27 in SVECs but not SVEC-vGPCR. Induction of VDR was blocked by transfection of short hairpinRNAagainst VDR in SVEC-vGPCR and the antiproliferative effects of 1α,25(OH)2D3 and TX527 were decreased, involving the VDR genomic pathway in the hormone and analog mechanism of action. In vivo experiments showed that 1α,25(OH)2D3 and TX527 decreased SVEC-vGPCR tumor progression when the tumor cells were implanted in nude mice. In conclusion, we have demonstrated that 1α,25(OH) 2D3 and its TX527 analog have antiproliferative effects on the growth of endothelial cells transformed by the vGPCR in vitro and in vivo, the vitamin D receptor being part of the inhibitory mechanism of action. Copyright © 2010 by The Endocrine Society.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
1a, 25(Oh)2 D3
dc.subject
Tx527 Analog
dc.subject
Vdr
dc.subject
Cell Proliferation
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
1α,25-dihydroxyvitamin D3 and its TX527 analog inhibit the growth of endothelial cells transformed by Kaposi sarcoma-associated herpes virus G protein-coupled receptor in vitro and in vivo
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-18T15:02:40Z
dc.journal.volume
151
dc.journal.number
1
dc.journal.pagination
23-31
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur; Argentina
dc.description.fil
Fil: Martin, Daniel. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Gutkind, J. Silvio. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Verstuyf, Annemieke. Katholieke Universiteit Leuven; Bélgica
dc.description.fil
Fil: Bouillon, Roger. Katholieke Universiteit Leuven; Bélgica
dc.description.fil
Fil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur; Argentina
dc.description.fil
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur; Argentina
dc.journal.title
Endocrinology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1210/en.2009-0650
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article/151/1/23/2456029
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