Artículo
Isolation of a strawberry gene fragment encoding an actin depolymerizing factor-like protein from genotypes resistant to Colletotrichum acutatum
Ontivero, Marta; Martinez Zamora, Martin Gustavo
; Salazar, Sergio Miguel; Diaz Ricci, Juan Carlos
; Castagnaro, Atilio Pedro
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Fecha de publicación:
12/2011
Editorial:
National Research Council Canada-NRC Research Press
Revista:
Genome
ISSN:
0831-2796
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Actin depolymerizing factors (ADFs) have been recently implicated in plant defense against pathogenic fungi, associated with the cytoskeletal rearrangements that contribute to establish an effective barrier against fungal ingress. In this work, we identified a DNA fragment corresponding to a part of a gene predicted to encode an ADF-like protein in genotypes of Fragaria ananassa resistant to the fungus Colletotrichum acutatum. Bulked segregant analysis combined with AFLP was used to identify polymorphisms linked to resistance in hybrids derived from the cross between the resistant cultivar 'Sweet Charlie' and the susceptible cultivar 'Pájaro'. The sequence of one out of three polymorphic bands detected showed significant BLASTX hits to ADF proteins from other plants. Two possible exons were identified and bioinformatic analysis revealed the presence of the ADF homology domain with two actin-binding sites, an N-terminal phosphorylation site, and a nuclear localization signal. In addition to its possible application in strawberry breeding programs, these finding may contribute to investigate the role of ADFs in plant resistance against fungi.
Palabras clave:
Anthracnose
,
Fragaria Ananassa
,
Molecular Markers
,
Plant Disease Resistance
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Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Ontivero, Marta; Martinez Zamora, Martin Gustavo; Salazar, Sergio Miguel; Diaz Ricci, Juan Carlos; Castagnaro, Atilio Pedro; Isolation of a strawberry gene fragment encoding an actin depolymerizing factor-like protein from genotypes resistant to Colletotrichum acutatum; National Research Council Canada-NRC Research Press; Genome; 54; 12; 12-2011; 1041-1044
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