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dc.contributor.author
Ventura, Alejandra  
dc.contributor.author
Bruno, Luciana  
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Demuro, Angelo  
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Parker, Ian  
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Ponce Dawson, Silvina Martha  
dc.date.available
2018-09-28T14:21:01Z  
dc.date.issued
2005-12  
dc.identifier.citation
Ventura, Alejandra; Bruno, Luciana; Demuro, Angelo; Parker, Ian; Ponce Dawson, Silvina Martha; A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients; Cell Press; Biophysical Journal; 88; 4; 12-2005; 2403-2421  
dc.identifier.issn
0006-3495  
dc.identifier.uri
http://hdl.handle.net/11336/61174  
dc.description.abstract
Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca2+ flux. However, this is difficult to infer from fluorescence Ca2+ images because the distribution of Ca2+-bound dye is affected by poorly characterized processes including diffusion of Ca2+ ions, their binding to mobile and immobile buffers, and sequestration by Ca2+ pumps. Several methods have previously been proposed to derive Ca2+ flux from fluorescence images, but all require explicit knowledge or assumptions regarding these processes. We now present a novel algorithm that requires few assumptions and is largely model-independent. By testing the algorithm with both numerically generated image data and experimental images of sparklets resulting from Ca2+ flux through individual voltage-gated channels, we show that it satisfactorily reconstructs the magnitude and time course of the underlying Ca2+ currents. © 2005 by the Biophysical Society.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Cell Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-09-27T20:17:50Z  
dc.journal.volume
88  
dc.journal.number
4  
dc.journal.pagination
2403-2421  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Nueva York  
dc.description.fil
Fil: Ventura, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina  
dc.description.fil
Fil: Bruno, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina  
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Fil: Demuro, Angelo. University of California at Irvine; Estados Unidos  
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Fil: Parker, Ian. University of California at Irvine; Estados Unidos  
dc.description.fil
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina  
dc.journal.title
Biophysical Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1529/biophysj.104.045260