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dc.contributor.author
Navarrete, Felipe A.
dc.contributor.author
Alvau, Antonio
dc.contributor.author
Lee, Hoi Chang
dc.contributor.author
Levin, Lonny R.
dc.contributor.author
Buck, Jochen
dc.contributor.author
Leon, Patricia Martin De
dc.contributor.author
Santi, Celia M.
dc.contributor.author
Krapf, Dario
dc.contributor.author
Mager, Jesse
dc.contributor.author
Fissore, Rafael A.
dc.contributor.author
Salicioni, Ana M.
dc.contributor.author
Darszon, Alberto
dc.contributor.author
Visconti, Pablo E.
dc.date.available
2018-09-26T19:44:26Z
dc.date.issued
2016-09
dc.identifier.citation
Navarrete, Felipe A.; Alvau, Antonio; Lee, Hoi Chang; Levin, Lonny R.; Buck, Jochen; et al.; Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models; Nature Publishing Group; Scientific Reports; 6; 9-2016; 1-9; 33589
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/60986
dc.description.abstract
Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca 2+ ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function. We now show that a pulse of Ca2+ ionophore induces fertilizing capacity in sperm from infertile CatSper1 (Ca2+ channel), Adcy10 (soluble adenylyl cyclase) and Slo3 (K+ channel) KO mice. In contrast, sperm from infertile mice lacking the Ca 2+ efflux pump PMACA4 were not rescued. These results indicate that a transient increase in intracellular Ca 2+ can overcome genetic infertility in mice and suggest this approach may prove adaptable to rescue sperm function in certain cases of human male infertility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Calcium
dc.subject
Fertilization
dc.subject
Fertility
dc.subject
Ionophore
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-26T14:58:26Z
dc.journal.number
6
dc.journal.pagination
1-9; 33589
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Navarrete, Felipe A.. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Alvau, Antonio. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Lee, Hoi Chang. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Levin, Lonny R.. Weill Cornell Medical College; Estados Unidos
dc.description.fil
Fil: Buck, Jochen. Weill Cornell Medical College; Estados Unidos
dc.description.fil
Fil: Leon, Patricia Martin De. University of Delaware; Estados Unidos
dc.description.fil
Fil: Santi, Celia M.. University of Washington; Estados Unidos
dc.description.fil
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Mager, Jesse. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Fissore, Rafael A.. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Salicioni, Ana M.. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
dc.description.fil
Fil: Visconti, Pablo E.. University of Massachusetts; Estados Unidos
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/srep33589
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep33589


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    Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO

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