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dc.contributor.author
Molinas, Sara Maria
dc.contributor.author
Soria, Leandro Raul
dc.contributor.author
Marrone, Julieta
dc.contributor.author
Danielli, Mauro
dc.contributor.author
Trumper, Laura
dc.contributor.author
Marinelli, Raul Alberto
dc.date.available
2016-06-08T13:26:19Z
dc.date.issued
2015-05
dc.identifier.citation
Molinas, Sara Maria; Soria, Leandro Raul; Marrone, Julieta; Danielli, Mauro; Trumper, Laura; et al.; Acidosis-induced downregulation of hepatocyte mitochondrial aquaporin-8 and ureagenesis from ammonia; Canadian Science Publishing; Biochemistry and Cell Biology; 93; 4; 5-2015; 417-420
dc.identifier.issn
0829-8211
dc.identifier.uri
http://hdl.handle.net/11336/6096
dc.description.abstract
It has been proposed that during metabolic acidosis, the liver downregulates mitochondrial ammonia detoxification via ureagenesis, a bicarbonate-consuming process. Since we previously demonstrated that hepatocyte mitochondrial aquaporin-8 channels (mtAQP8) facilitate the uptake of ammonia and its metabolism into urea, we studied whether mtAQP8 is involved in the liver adaptive response to acidosis. Primary cultured rat hepatocytes were adapted to acidosis by exposing them to culture medium at pH 7.0 for 40 h. Control cells were exposed to pH 7.4. Hepatocytes exposed to acid medium showed a decrease in mtAQP8 protein expression (-30%, p<0.05). Ureagenesis from ammonia was assessed by incubating the cells with 15N-labeled ammonia and measuring 15N-labeled urea synthesis by nuclear magnetic resonance. Reduced ureagenesis was found in acidified hepatocytes (-31%, p<0.05). In vivo studies in rats subjected to 7-days acidosis also showed decreased protein expression of hepatic mtAQP8 (-50%, p<0.05) and reduced liver urea content (-35%; p<0.05). In conclusion, our in vitro and in vivo data suggest that hepatic mtAQP8 expression is downregulated in acidosis, a mechanism that may contribute to decreased ureagenesis from ammonia in response to acidosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Canadian Science Publishing
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Aquaporin 8
dc.subject
Hepatocyte
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Mitochondrial Ammonia Transport
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Acidosis
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Ureagenesis
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Fisiología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Acidosis-induced downregulation of hepatocyte mitochondrial aquaporin-8 and ureagenesis from ammonia
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-06-01T14:05:04Z
dc.journal.volume
93
dc.journal.number
4
dc.journal.pagination
417-420
dc.journal.pais
Canadá
dc.journal.ciudad
Otawa
dc.description.fil
Fil: Molinas, Sara Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
dc.description.fil
Fil: Soria, Leandro Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
dc.description.fil
Fil: Marrone, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
dc.description.fil
Fil: Danielli, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
dc.description.fil
Fil: Trumper, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil
Fil: Marinelli, Raul Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
dc.journal.title
Biochemistry and Cell Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/bcb-2014-0168
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1139/bcb-2014-0168
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/bcb-2014-0168


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