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dc.contributor.author
Raiger Iustman, Laura Judith
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dc.contributor.author
Ruiz, Jimena
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dc.date.available
2018-09-26T15:44:21Z
dc.date.issued
2008-07
dc.identifier.citation
Raiger Iustman, Laura Judith; Ruiz, Jimena; The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 284; 2; 7-2008; 218-224
dc.identifier.issn
0378-1097
dc.identifier.uri
http://hdl.handle.net/11336/60880
dc.description.abstract
To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). By comparison with the wild-type, the rpoS mutant showed a higher polyhydroxyalkanoate degradation rate and increased expression of the translational fusion during the stationary growth phase. These results suggest that σS might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the accumulated polyhydroxyalkanoate increased the survival and stress tolerance of the rpoS mutant. According to this, polyhydroxyalkanoate accumulation would help cells to overcome the adverse conditions encountered during the stationary phase in the strain that lacks RpoS. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Polyhydroxyalkanoate
dc.subject
Pseudomonas Putida
dc.subject
Regulation
dc.subject
Rpos
dc.subject
Stress Resistance
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-21T20:36:48Z
dc.journal.volume
284
dc.journal.number
2
dc.journal.pagination
218-224
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Raiger Iustman, Laura Judith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Ruiz, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.journal.title
FEMS Microbiology Letters
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/j.1574-6968.2008.01203.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/284/2/218/516492
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