Mostrar el registro sencillo del ítem
dc.contributor.author
Vignaduzzo, Silvana Edit
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Kaufman, Teodoro Saul
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2016-06-06T21:22:22Z
dc.date.issued
2013-09
dc.identifier.citation
Vignaduzzo, Silvana Edit; Kaufman, Teodoro Saul; Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations; Taylor & Francis; Journal of Liquid Chromatography and Related Technologies; 36; 20; 9-2013; 2829-2843
dc.identifier.issn
1082-6076
dc.identifier.uri
http://hdl.handle.net/11336/6078
dc.description.abstract
A simple and efficient liquid chromatographic method has been developed and validated for the simultaneous determination of bromhexine, chlorpheniramine, paracetamol, and pseudoephedrine in common cold medications (tablets and syrups). The separation of the analytes was achieved within 10 min, employing a mixture of 10 mM triethylamine-phosphoric acid buffer (pH 4.0) and MeOH (35:65, v/v) as isocratic mobile phase, pumped at 1.0 mL min−1 through a cyano column (5 µm particle size). The analytes were detected at 215 nm. Statistical experimental designs and graphic representations (response surface methodologies, Pareto charts) were used for selecting the proper detection wavelength, optimizing the mobile phase composition, and assessing method robustness. The linearity of the calibration (r > 0.99, n = 21) in the relevant ranges (up to 130% of the expected concentrations of the analytes in the formulations), method accuracy (bias < 2.0%), repeatability (RSD < 2.0%) and intermediate precision, were verified. In addition, specificity (peak purities with photodiode array detector >0.9997) and method robustness were evaluated, and system suitability parameters were determined. The validated method was successfully employed for the routine analysis of various commercial tablet and syrup pharmaceutical preparations against the common cold, showing satisfactory analyte recoveries and RSD values.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bromhexine
dc.subject
Chlorpheniramine
dc.subject
Experimental Design
dc.subject
Paracetamol
dc.subject
Validated HPLC determination
dc.subject
Pseudoephedrine
dc.subject.classification
Química Analítica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Químicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Development and Validation of an HPLC Method for the Simultaneous Determination of Bromhexine, Chlorpheniramine, Paracetamol and Pseudoephedrine in their Combined Cold Medicine Formulations
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-06-01T13:33:44Z
dc.journal.volume
36
dc.journal.number
20
dc.journal.pagination
2829-2843
dc.journal.pais
Reino Unido
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Londres
dc.description.fil
Fil: Vignaduzzo, Silvana Edit. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil
Fil: Kaufman, Teodoro Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.journal.title
Journal of Liquid Chromatography and Related Technologies
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/10826076.2012.717055
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/10826076.2012.717055
Archivos asociados